Neural-competent cells of adult human dermis belong to the Schwann lineage.
Neural-competent cells of adult human dermis belong to the Schwann lineage.
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DOI：
10.1016/j.stemcr.2014.09.009
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发表时间：
2014-11-11
影响因子：
5.9
通讯作者：
Izeta, Ander
中科院分区：
文献类型：
--
作者：
Etxaniz, Usue;Perez-San Vicente, Adrian;Gago-Lopez, Nuria;Garcia-Dominguez, Mario;Iribar, Haizea;Aduriz, Ariane;Perez-Lopez, Virginia;Burgoa, Izaskun;Irizar, Haritz;Munoz-Culla, Maider;Vallejo-Illarramendi, Ainara;Leis, Olatz;Matheu, Ander;Martin, Angel G.;Otaegui, David;Paz Lopez-Mato, Maria;Gutierrez-Rivera, Araika;MacLellan, Robb;Izeta, Ander
据报道，许多成人组织中存在神经前体细胞（NPC）。了解它们的生理功能，或者在组织损伤或体外操作后它们的激活仍然是一个未解决的问题。在这里，我们调查了成人组织中人真皮NPC的来源。通过采用细胞表面标记物筛选、细胞分离、转录组学表征和体内命运分析的无偏见、全面的方法，我们发现人包皮的p75NTR+前体可归因于Schwann（CD56+）和血管周围（CD56−）细胞谱系。此外，神经分化潜能仅限于p75NTR+CD56+ Schwann细胞，并由SOX 2表达水平介导。从人心脏球中同样获得双阳性NPC，表明这种现象可能很普遍。人真皮来源的培养物显示两种类型的SOX 2+细胞：雪旺氏细胞和血管周围p75NTR+ CD 56+雪旺氏细胞负责神经子代SOX 2表达水平调节真皮前体的神经能力p75NTR+ CD 56+神经前体细胞类似地来自人心脏球在这篇文章中，Izeta及其同事表明，来自成人真皮的神经前体细胞（NPC）培养物包括两个SOX 2+细胞亚群。（雪旺氏细胞和血管周围细胞），并且神经子代仅在由其SOX 2表达水平调节的过程中源自雪旺氏细胞。NPC同样来自人类心脏球，可能阐明在多个器官中观察到的看似无关的NPC的起源。
Resident neural precursor cells (NPCs) have been reported for a number of adult tissues. Understanding their physiological function or, alternatively, their activation after tissue damage or in vitro manipulation remains an unsolved issue. Here, we investigated the source of human dermal NPCs in adult tissue. By following an unbiased, comprehensive approach employing cell-surface marker screening, cell separation, transcriptomic characterization, and in vivo fate analyses, we found that p75NTR+ precursors of human foreskin can be ascribed to the Schwann (CD56+) and perivascular (CD56−) cell lineages. Moreover, neural differentiation potential was restricted to the p75NTR+CD56+ Schwann cells and mediated by SOX2 expression levels. Double-positive NPCs were similarly obtained from human cardiospheres, indicating that this phenomenon might be widespread. Human dermis-derived cultures show two types of SOX2+ cells: Schwann and perivascular p75NTR+CD56+ Schwann cells are responsible for neural progeny SOX2 expression levels regulate the neural competence of dermal precursors p75NTR+CD56+ neural precursor cells similarly arise from human cardiospheres In this article, Izeta and colleagues show that neural precursor cell (NPC) cultures from adult human dermis include two SOX2+ cell subpopulations (Schwann and perivascular), and neural progeny is derived only from Schwann cells in a process regulated by their SOX2 expression levels. NPCs similarly arise from human cardiospheres, potentially elucidating the origin of seemingly unrelated NPCs observed in multiple organs.
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影响因子：
2.7
作者：
Hutton, Scott R.;Pevny, Larysa H.
通讯作者：
Pevny, Larysa H.
影响因子：
21.3
作者：
Fernandes, KJL;McKenzie, IA;Miller, FD
通讯作者：
Miller, FD
影响因子：
23.9
作者：
Karow, Marisa;Sanchez, Rodrigo;Berninger, Benedikt
通讯作者：
Berninger, Benedikt
影响因子：
64.8
作者：
通讯作者：
--
影响因子：
5.9
作者：
Krause, Matthew P.;Dworski, Shaalee;Feinberg, Konstantin;Jones, Karen;Johnston, Adam P. W.;Paul, Smitha;Paris, Maryline;Peles, Elior;Bagli, Darius;Forrest, Christopher R.;Kaplan, David R.;Miller, Freda D.
通讯作者：
Miller, Freda D.