Orexin signaling in rostral lateral hypothalamus and nucleus accumbens shell in the control of spontaneous physical activity in high- and low-activity rats.
Orexin signaling in rostral lateral hypothalamus and nucleus accumbens shell in the control of spontaneous physical activity in high- and low-activity rats.
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下丘脑头侧外侧和伏核壳中的食欲素信号传导控制高活动和低活动大鼠的自发身体活动。
DOI：
10.1152/ajpregu.00339.2016
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
Kotz,CatherineM
中科院分区：
文献类型：
--
作者：
Perez-Leighton,Claudio;Little,MorganR;Grace,Martha;Billington,Charles;Kotz,CatherineM
自发性体力活动(SPA)描述的是正式锻炼以外的活动，并表现出很大的个体间变异性。下丘脑增食欲素/下克汀肽是SPA的关键调节因子。食欲素在多个大脑部位驱动SPA，包括头侧下丘脑(Lh)和伏隔核外壳(NAcSh)。与低活动(LA)大鼠相比，高基础SPA(高活性，HA)大鼠在头端LH区注射食欲素-A后，Oresin-A的表达和SPA显著增加。在这里，我们探讨了在嘴的LH和NAcSh的食欲素信号对HA/LA表型的贡献。我们发现，在头侧LH区注射增食欲素-A后，HA大鼠对SPA有较高的敏感性，而在NAcSh区则不敏感。HA和LA大鼠在头端LH区表现出相似水平的食欲素受体表达，注射食欲素-A后，产生食欲素的神经元在头端LH区被激活。此外，在HA和LA大鼠中，在头端LH区和NAcSh区共同注射食欲素-A并不能进一步增加SPA，而不是在嘴部LH区注射食欲素-A的影响。NAcSh内预先给予GABA受体激动剂麝香酚，可增强HA大鼠在头侧LH区注射增食欲素A产生的SPA，但对LA大鼠无明显影响。我们的结果表明，从头端LH区的食欲素反应神经元到食欲素神经元的反馈环和NAcSh-食欲素神经元-头端LH环调节SPA。总体而言，我们的数据表明，头端促黄体生成素对食欲素敏感性的差异以及来自NAcSh的GABA传入对其调节的差异导致了个体SPA的差异。
Spontaneous physical activity (SPA) describes activity outside of formal exercise and shows large interindividual variability. The hypothalamic orexin/hypocretin peptides are key regulators of SPA. Orexins drive SPA within multiple brain sites, including rostral lateral hypothalamus (LH) and nucleus accumbens shell (NAcSh). Rats with high basal SPA (high activity, HA) show higher orexin mRNA expression and SPA after injection of orexin-A in rostral LH compared with low-activity (LA) rats. Here, we explored the contribution of orexin signaling in rostral LH and NAcSh to the HA/LA phenotype. We found that HA rats have higher sensitivity to SPA after injection of orexin-A in rostral LH, but not in NAcSh. HA and LA rats showed similar levels of orexin receptor expression in rostral LH, and activation of orexin-producing neurons after orexin-A injection in rostral LH. Also, in HA and LA rats, the coinjection of orexin-A in rostral LH and NAcSh failed to further increase SPA beyond the effects of orexin-A in rostral LH. Pretreatment with muscimol, a GABAAreceptor agonist, in NAcSh potentiated SPA produced by orexin-A injection in rostral LH in HA but not in LA rats. Our results suggest that a feedback loop from orexin-responsive neurons in rostral LH to orexin neurons and a the NAcSh–orexin neuron–rostral LH circuit regulate SPA. Overall, our data suggest that differences in orexin sensitivity in rostral LH and its modulation by GABA afferents from NAcSh contribute to individual SPA differences.
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影响因子：
5.8
作者：
R. Scharpf;R. Irizarry;Matthew E. Ritchie;B. Carvalho;I. Ruczinski
通讯作者：
R. Scharpf;R. Irizarry;Matthew E. Ritchie;B. Carvalho;I. Ruczinski
下丘脑外侧的下丘脑分泌素 I 激活关键的进食调节大脑部位
影响因子：
1.7
作者：
M. Mullett;C. Billington;A. Levine;C. Kotz
通讯作者：
C. Kotz
DOI：
10.1152/ajpendo.00126.2003
发表时间：
2004-04-01
影响因子：
5.1
作者：
Kiwaki, K;Kotz, CM;Levine, JA
通讯作者：
Levine, JA
影响因子：
3.7
作者：
A. N. Acad;Sci;C. Kotz;J. Nixon;T. Butterick;C. Perez-Leighton;J. Teske;C. Billington
通讯作者：
A. N. Acad;Sci;C. Kotz;J. Nixon;T. Butterick;C. Perez-Leighton;J. Teske;C. Billington
DOI：
10.1073/pnas.95.1.322
发表时间：
1998-01-06
影响因子：
11.1
作者：
De Lecea, L;Kilduff, TS;Sutcliffe, JG
通讯作者：
Sutcliffe, JG