Stem cell treatment improves post stroke neurological outcomes: a comparative study in male and female rats.
Stem cell treatment improves post stroke neurological outcomes: a comparative study in male and female rats.
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DOI：
10.1136/svn-2020-000834
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发表时间：
2021-12
影响因子：
5.9
通讯作者：
Veeravalli KK
中科院分区：
文献类型：
--
作者：
Nalamolu KR;Chelluboina B;Fornal CA;Challa SR;Pinson DM;Wang DZ;Klopfenstein JD;Veeravalli KK
不同干细胞治疗缺血性中风的潜力令人着迷，但也存在一些争议。我们实验室的最新结果证明了人类脐带血来源的间充质干细胞 (MSC) 在啮齿动物中风模型中的潜在益处。我们假设间充质干细胞治疗将有效促进男性和女性感觉和运动功能的恢复，尽管中风后脑损伤存在明显的性别差异。通过闭塞大脑中动脉，在成年 Sprague-Dawley 大鼠中诱导短暂性局灶性脑缺血。手术结束后，再灌注1天后，未治疗组的雄性和雌性大鼠被安乐死，并用它们的大脑来估计由此产生的梗塞体积和组织肿胀。另外一组中风诱发的雄性和雌性大鼠接受 MSC 或载体治疗，随后接受一系列标准神经学/神经行为测试（改良神经学严重程度评分评估、胶带去除、平衡木步行和旋转棒）。再灌注后定期（第 1、3、5、7 和 14 天）进行测试，以确定中风后神经和功能恢复的时间过程。男性和女性的梗塞体积和缺血性脑肿胀程度相似。尽管病理性中风病变相似，但神经系统评分和其他测试表明，男性中风的临床表现比女性更明显。间充质干细胞治疗显着改善了男女感觉和运动功能的恢复，并且对中度中风（女性）和重度中风（男性）均显示出疗效。尽管中风后结果的严重程度存在性别差异，但间充质干细胞治疗促进了雄性和雌性大鼠感觉和运动功能的恢复，这表明它可能是一种有前途的中风治疗方法。
The therapeutic potential of different stem cells for ischaemic stroke treatment is intriguing and somewhat controversial. Recent results from our laboratory have demonstrated the potential benefits of human umbilical cord blood-derived mesenchymal stem cells (MSC) in a rodent stroke model. We hypothesised that MSC treatment would effectively promote the recovery of sensory and motor function in both males and females, despite any apparent sex differences in post stroke brain injury. Transient focal cerebral ischaemia was induced in adult Sprague-Dawley rats by occlusion of the middle cerebral artery. Following the procedure, male and female rats of the untreated group were euthanised 1 day after reperfusion and their brains were used to estimate the resulting infarct volume and tissue swelling. Additional groups of stroke-induced male and female rats were treated with MSC or vehicle and were subsequently subjected to a battery of standard neurological/neurobehavioral tests (Modified Neurological Severity Score assessment, adhesive tape removal, beam walk and rotarod). The tests were administered at regular intervals (at days 1, 3, 5, 7 and 14) after reperfusion to determine the time course of neurological and functional recovery after stroke. The infarct volume and extent of swelling of the ischaemic brain were similar in males and females. Despite similar pathological stroke lesions, the clinical manifestations of stroke were more pronounced in males than females, as indicated by the neurological scores and other tests. MSC treatment significantly improved the recovery of sensory and motor function in both sexes, and it demonstrated efficacy in both moderate stroke (females) and severe stroke (males). Despite sex differences in the severity of post stroke outcomes, MSC treatment promoted the recovery of sensory and motor function in male and female rats, suggesting that it may be a promising treatment for stroke.
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