The receptor genes PfBMPR1B and PfBAMBI are involved in regulating shell biomineralization in the pearl oyster Pinctada fucata.
The receptor genes PfBMPR1B and PfBAMBI are involved in regulating shell biomineralization in the pearl oyster Pinctada fucata.
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受体基因 PfBMPR1B 和 PfBAMBI 参与调节珠母贝贝壳生物矿化
DOI：
10.1038/s41598-017-10011-y
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发表时间：
2017-08-23
影响因子：
4.6
通讯作者：
Zhang R
中科院分区：
文献类型：
--
作者：
Li S;Liu Y;Huang J;Zhan A;Xie L;Zhang R
越来越多的证据表明，TGFβ/BMP信号通路最有可能参与软体动物贝壳的生物矿化，但对该通路受体的功能研究较少。本研究克隆并鉴定了合浦珠母贝（Pinctada fucata）的两个BMP受体基因PfBMPR 1和PfBAMBI。实时荧光定量PCR和原位杂交结果显示，这些基因表达于外套膜边缘和套膜，特异性定位于外上皮。利用RNA干扰（RNAi）技术敲低PfBMPR 1B基因可显著降低基质蛋白（MP）基因的表达水平，并诱导棱柱层和珍珠层的超微结构异常。相反，PfBAMBI的敲除显著增加了部分MP基因的表达水平，并诱导了naclonal层晶体的过度生长。在RNAi和壳缺口实验中，MP基因的表达受PfBMPR 1和PfBAMBI的竞争性调控。此外，受体抑制剂LDN 193189降低了MP基因在外套膜原代细胞和幼虫中的表达水平，并诱导幼虫发育过程中异常的D形壳形成。总之，这些结果清楚地表明PfBMPR 1和PfBAMBI参与调节P. fucata的壳生物矿化。因此，我们的研究提供了直接的证据，BMP受体参与软体动物的生物矿化。
Mounting evidence suggests that TGFβ/BMP signaling pathway is most likely involved in shell biomineralization in molluscs, but the function of pathway receptors is poorly studied. Here, we cloned and identified two homologous BMP receptor genes,PfBMPR1BandPfBAMBI, from the pearl oysterPinctada fucata. Real-time quantitative PCR andin situhybridization revealed that these genes were expressed in mantle edge and pallial, specifically located at the outer epithelia. Knockdown ofPfBMPR1Bby RNA interference (RNAi) significantly decreased the expression levels of matrix protein (MP) genes and induced the abnormal ultrastructure of prismatic and nacreous layers. Conversely, knockdown ofPfBAMBIsignificantly increased the expression levels of a portion of MP genes and induced the overgrowth of nacreous layer crystals. In the RNAi and shell notching experiments, MP gene expressions were competitively regulated byPfBMPR1BandPfBAMBI. In addition, the receptor inhibitor LDN193189 reduced the expression levels of MP genes in mantle primary cells and larvae, and induced abnormal D-shaped shell formation during larval development. Collectively, these results clearly show thatPfBMPR1BandPfBAMBIare involved in regulating shell biomineralization inP.fucata. Our study therefore provides the direct evidence that BMP receptors participate in mollusc biomineralization.
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