Bottom-up signaling from HGF-containing surfaces promotes hepatic differentiation of mesenchymal stem cells.
Bottom-up signaling from HGF-containing surfaces promotes hepatic differentiation of mesenchymal stem cells.
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DOI：
10.1016/j.bbrc.2011.03.005
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发表时间：
2011-04-08
影响因子：
3.1
通讯作者：
Revzin, Alexander
中科院分区：
文献类型：
--
作者：
Ghaedi, Mahboobe;Tuleuova, Nazgul;Zern, Mark A.;Wu, Jian;Revzin, Alexander
关键词：
干细胞分化为特定细胞类型的能力使其在组织再生和修复方面非常有前途。然而，实现这一前景需要新的方法来指导干细胞的特定谱系分化。在这项研究中，肝细胞生长因子(HGF)是肝脏发育中的重要形态因子，与I型胶原(Col)共打印在玻璃上形成蛋白质斑点阵列。人脂肪干细胞(ASCs)在HGF/Col斑点上培养2周。采用逆转录聚合酶链式反应、酶联免疫吸附试验和免疫细胞化学方法分析表面固定化HGF对ASCs肝脏分化的影响。通过免疫细胞化学和酶联免疫吸附试验检测，自下而上用肝细胞生长因子刺激干细胞可引起α-胎儿蛋白和白蛋白合成的上调。RT-PCR结果显示，在肝细胞生长因子/COL阵列上培养的干细胞中白蛋白、α胎蛋白和α-1抗胰蛋白酶的表达水平是仅在COL斑点上干细胞的10~20倍。我们的结果表明，含有HGF和ECM蛋白共印迹的表面可以用于将ASCs等间充质干细胞分化为肝细胞样细胞。这些结果强调了含有生长因子的培养表面对干细胞分化的作用。
The capacity of stem cells to differentiate into specific cell types makes them very promising in tissue regeneration and repair. However, realizing this promise requires novel methods for guiding lineage-specific differentiation of stem cells. In this study, hepatocyte growth factor (HGF), an important morphogen in liver development, was co-printed with collagen I (Col) to create arrays of protein spots on glass. Human adipose stem cells (ASCs) were cultured on top of the HGF/Col spots for 2 weeks. The effects of surface-immobilized HGF on hepatic differentiation of ASCs were analyzed using RT-PCR, ELISA and immunocytochemistry. Stimulation of stem cells with HGF from the bottom-up caused an upregulation in synthesis of α-fetoprotein and albumin, as determined by immunocytochemistry and ELISA. RT-PCR results showed that the mRNA levels for albumin, α-fetoprotein and α1 antitrypsin were 10 to 20 fold higher in stem cells cultured on the HGF/Col arrays compared to stem cells on Col only spots. Our results show that surfaces containing HGF co-printed with ECM proteins may be used to differentiate mesenchymal stem cells such as ASCs into hepatocyte-like cells. These results underscore the utility of growth factor-containing culture surfaces for stem cell differentiation.
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影响因子：
14
作者：
Curcio, Efrern;Salerno, Simona;Bader, Augustinus
通讯作者：
Bader, Augustinus
影响因子：
2.7
作者：
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影响因子：
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影响因子：
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影响因子：
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作者：
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通讯作者：
West, JL