Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees.
Development of Dot-ELISA and Colloidal Gold Immunochromatographic Strip for Rapid and Super-Sensitive Detection of Plum Pox Virus in Apricot Trees.
复制标题
开发用于快速超灵敏检测杏树李痘病毒的斑点酶联免疫吸附测定和胶体金免疫层析试纸条
DOI：
10.3390/v15010169
复制
发表时间：
2023-01-05
期刊：
影响因子：
--
通讯作者：
Wu J
中科院分区：
文献类型：
--
作者：
Guo M;Qi D;Dong J;Dong S;Yang X;Qian Y;Zhou X;Wu J
李痘病毒是核果类果树夏卡病的病原，常造成巨大的经济损失。由于其在世界范围内的分布和经济重要性，快速和可靠的诊断技术对于成功管理鲨鱼病变得越来越重要。在本研究中，我们制备了两种超敏感和特异的抗PPV单克隆抗体（即，MAb 13 H4和4A 11）。使用这两种单克隆抗体，我们现在已经开发了一种斑点酶联免疫吸附试验（dot-ELISA）和胶体金免疫层析条（CGICS）测定。这两种技术可用于快速可靠地检测PPV。这些敏感性试验的结果证实，dot-ELISA和CGICS试验可以检测到PPV感染杏树叶粗提物稀释至1：5120和1：6400（w/v），分别。进一步分析表明，斑点ELISA的检测终点是通过RT-PCR获得的检测终点的约26倍，CGICS与RT-PCR一样敏感。本研究旨在拓宽对dot-ELISA和CGICS检测PPV的检出限的认识。我们认为，这些新开发的dot-ELISA和CGICS是特别有用的大规模PPV调查领域。
Plum pox virus (PPV) is a causal agent of the stone fruit tree sharka disease that often causes enormous economic losses. Due to its worldwide distribution and economic importance, rapid and reliable diagnostic technologies are becoming increasingly important for successful management of sharka disease. In this study, we have produced two super-sensitive and specific anti-PPV monoclonal antibodies (i.e., MAbs 13H4 and 4A11). Using these two MAbs, we have now developed a dot enzyme-linked immunosorbent assay (dot-ELISA) and a colloidal gold immunochromatographic strip (CGICS) assay. These two technologies can be used to quickly and reliably detect PPV. The results of these sensitivity assays confirmed that the dot-ELISA and CGICS assays could detect PPV infection in apricot tree leaf crude extracts diluted up to 1:5120 and 1:6400 (w/v), respectively. Further analyses using field-collected apricot tree leaf samples showed that the detection endpoint of the dot-ELISA was ~26 times above that obtained through RT-PCR, and the CGICS was as sensitive as RT-PCR. This present study is to broaden the knowledge about detection limits of dot-ELISA and CGICS for PPV monitoring. We consider that these newly developed dot-ELISA and CGICS are particularly useful for large scale PPV surveys in fields.
登录
查看更多内容
影响因子：
1.8
作者：
Mori T;Warner C;Ohno S;Mori K;Tobimatsu T;Sera T
通讯作者：
Sera T
影响因子：
5.5
作者：
Liu, Zhen;Chen, Zhe;Wu, Jianxiang
通讯作者：
Wu, Jianxiang
影响因子：
1.8
作者：
Mori T;Takenaka K;Domoto F;Aoyama Y;Sera T
通讯作者：
Sera T
影响因子：
1.4
作者：
Elibuyuk, I. Ozer
通讯作者：
Elibuyuk, I. Ozer
三种基于单克隆抗体的高灵敏度血清学检测方法检测番茄斑驳花叶病毒
影响因子：
3.4
作者：
Li, Xue;Guo, Liqian;Wu, Jianxiang
通讯作者：
Wu, Jianxiang