Genome sequence analysis of new plum pox virus isolates from Japan.
Genome sequence analysis of new plum pox virus isolates from Japan.
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DOI：
10.1186/s13104-021-05683-9
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发表时间：
2021-07-10
影响因子：
1.8
通讯作者：
Sera T
中科院分区：
文献类型：
--
作者：
Mori T;Warner C;Ohno S;Mori K;Tobimatsu T;Sera T
为了发现最近可能发生在李痘病毒（PPV）的突变，我们收集了六个PPV感染的李/桃树从日本西部和东部的一部分。在对全长PPV基因组RNA进行测序后，我们将氨基酸序列与每种PPV毒株的代表性分离株进行了比较。发现所有新分离株均属于PPV-D株：从日本西部采集的6株分离株被鉴定为西日本株，而从日本东部采集的1株被鉴定为东日本株。氨基酸序列分析表明，这7个分离株的第1407和1529位氨基酸残基分别是西日本和东日本株的特征。将它们与47个非日本PPV-D分离株的相应氨基酸残基进行比较，发现这些氨基酸残基无疑是独特的。对在日本收集的其他210株PPV-D分离株的相关氨基酸残基进行进一步检查，产生了关于海外入侵途径和随后在日本境内扩散途径的新假设：PPV-D毒株可能从海外入侵日本西部并在日本全境传播。在线版本包含补充材料，可通过10.1186/s13104-021-05683-9获得。
To find mutations that may have recently occurred in Plum pox virus (PPV), we collected six PPV-infected plum/peach trees from the western part of Japan and one from the eastern part. After sequencing the full-length PPV genomic RNAs, we compared the amino acid sequences with representative isolates of each PPV strain. All new isolates were found to belong to the PPV-D strain: the six isolates collected from western Japan were identified as the West-Japan strain while the one collected from eastern Japan as the East-Japan strain. Amino acid sequence analysis of these seven isolates suggested that the 1407th and 1529th amino acid residues are characteristic of the West-Japan and the East-Japan strains, respectively. Comparing them with the corresponding amino acid residues of the 47 non-Japanese PPV-D isolates revealed that these amino acid residues are undoubtedly unique. A further examination of the relevant amino acid residues of the other 210 PPV-D isolates collected in Japan generated a new hypothesis regarding the invasion route from overseas and the subsequent diffusion route within Japan: a PPV-D strain might have invaded the western part of Japan from overseas and spread throughout Japan. The online version contains supplementary material available at 10.1186/s13104-021-05683-9.
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影响因子：
10.7
作者：
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通讯作者：
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影响因子：
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