Molecularly imprinted polymer for selective extraction and simultaneous determination of four tropane alkaloids from Przewalskia tangutica Maxim. fruit extracts using LC-MS/MS
Molecularly imprinted polymer for selective extraction and simultaneous determination of four tropane alkaloids from Przewalskia tangutica Maxim. fruit extracts using LC-MS/MS
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分子印迹聚合物用于选择性提取并同时测定普氏原木中的四种托烷生物碱。
DOI：
10.1039/c5ra18608k
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发表时间：
2015-11
期刊：
影响因子：
3.9
通讯作者：
Wang, Shanshan
中科院分区：
文献类型：
--
作者：
Ma, Xinbing;Jin, Maojun;Jin, Fen;Wang, Shanshan
以樟柳碱（ASD）为模板分子，甲基丙烯酸（MAA）为功能单体，三甲基丙烯酸异丙酯（TRIM）为交联剂，乙腈为致孔剂，采用沉淀聚合法制备了一种可选择性提取和富集4种托烷类生物碱的分子印迹聚合物（MIP）。通过选择性识别能力、吸附等温线和吸附动力学等方面对分子印迹聚合物和非分子印迹聚合物的性能进行了评价。结果表明，该分子印迹聚合物对TA具有明显的特异性识别作用，且吸附容量较大。为了验证分子印迹聚合物在真实的样品制备中的可行性，将所制备的分子印迹聚合物作为选择性吸附剂，对唐古特普氏原羚中的4种TA进行固相萃取。在优化的条件下，建立了一种快速、简便、高效的测定唐古特普氏原羚中4种总生物碱含量的方法。水果样品建立类特异性MIPs基于MISPE耦合高效液相色谱串联质谱法（LC-MS/MS）。该方法的回收率为82.1-102%，精密度RSD <5%，n = 5。水果提取物加标三个浓度水平（50、100、200 μg L−1）。结果表明，MIP-SPE可有效分离纯化唐古特普氏原羚粗提物中的总生物碱。
A class-specific molecularly imprinted polymer (MIP) for selective extraction and preconcentration of four tropane alkaloids has been successfully prepared by precipitation polymerization using anisodine (ASD) as the template, methacrylic (MAA) as the functional monomer, trimethylolpropane trimethacrylate (TRIM) as the crosslinker and acetonitrile as the porogen. The performances of the MIPs and the non-molecularly imprinted polymers (NIPs) were evaluated in terms of selective recognition capacity, adsorption isotherms and adsorption kinetics. The results indicated that the MIPs exhibited significant specific recognition toward TAs with a large adsorption capacity. To testify the feasibility of MIP in real sample preparation, the obtained MIPs were applied as the selective sorbents for the solid-phase extraction of four TAs in Przewalskia tangutica Maxim. Under optimized conditions, a rapid, convenient and efficient method for the determination of four TAs in Przewalskia tangutica Maxim. fruit samples was established by class-specific MIPs based MISPE coupling with high performance liquid chromatography tandem mass spectrometry (LC-MS/MS). The method gave excellent recoveries (82.1–102%) and precision (RSDs < 5%, n = 5) for Przewalskia tangutica Maxim. fruit extracts spiked at three concentration levels (50, 100, 200 μg L−1). The results demonstrated that the TAs in the extracts could be separated and purified through MIP-SPE from Przewalskia tangutica Maxim.
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影响因子：
--
作者：
Lan Xiaozhong;Q. Hong
通讯作者：
Lan Xiaozhong;Q. Hong
影响因子：
3.9
作者：
Shoulian Wei;Yong Liu;Zijun Yan;Ling Liu
通讯作者：
Shoulian Wei;Yong Liu;Zijun Yan;Ling Liu
DOI：
10.1016/j.chroma.2010.02.068
发表时间：
2010-04
期刊：
Journal of chromatography. A
影响因子：
--
作者：
David De Smet;S. Monbaliu;P. Dubruel;C. V. Van Peteghem;E. Schacht;S. de Saeger
通讯作者：
David De Smet;S. Monbaliu;P. Dubruel;C. V. Van Peteghem;E. Schacht;S. de Saeger
DOI：
10.1016/s0021-9673(02)02048-4
发表时间：
2003-02
期刊：
Journal of chromatography. A
影响因子：
--
作者：
G. Theodoridis;Andreas Kantifes;P. Manesiotis;N. Raikos;H. Tsoukali-Papadopoulou
通讯作者：
G. Theodoridis;Andreas Kantifes;P. Manesiotis;N. Raikos;H. Tsoukali-Papadopoulou
DOI：
--
发表时间：
2002-08
期刊：
Acta Botanica Sinica
影响因子：
--
作者：
Yang Dong-zhi;Zhang Zhi-yun;Lu An-ming;S. Kun;Li Jian-quan
通讯作者：
Yang Dong-zhi;Zhang Zhi-yun;Lu An-ming;S. Kun;Li Jian-quan