The Fall Armyworm Spodoptera frugiperda Utilizes Specific UDP-Glycosyltransferases to Inactivate Maize Defensive Benzoxazinoids.
The Fall Armyworm Spodoptera frugiperda Utilizes Specific UDP-Glycosyltransferases to Inactivate Maize Defensive Benzoxazinoids.
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DOI：
10.3389/fphys.2020.604754
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发表时间：
2020
影响因子：
4
通讯作者：
Vassão DG
中科院分区：
文献类型：
--
作者：
Israni B;Wouters FC;Luck K;Seibel E;Ahn SJ;Paetz C;Reinert M;Vogel H;Erb M;Heckel DG;Gershenzon J;Vassão DG
植物和昆虫之间的关系不断发展，许多昆虫依靠生化策略来减轻其食物植物中有毒化学物质的影响，使它们能够以防御良好的植物为食。草地贪夜蛾（Spodoptera frugiperda），秋季粘虫（FAW），接受许多植物作为宿主，并且在禾本科植物如玉米上特别成功，尽管它们具有苯并恶嗪类（BXD）防御。BXD作为惰性糖苷储存，在食草动物中被植物葡糖苷酶转化为有毒的糖苷配基。DIMBOA是玉米叶释放的主要BXD糖苷配基，可通过昆虫肠道中的UDP-糖基转移酶（UGT）立体选择性地进行再葡萄糖基化，使其无毒。在这里，我们确定UGT参与BXD解毒FAW幼虫和研究如何RNAi介导的操纵幼虫的葡萄糖基化能力对主要的玉米BXD，DIMBOA，影响幼虫的生长。我们的研究结果强调了两个主要的UGT家族UGT 33和UGT 40的成员参与BXD的糖基化。粪便中的大多数BXD排泄物以葡萄糖基化产物的形式发生。此外，DIMBOA相关活性在肠道组织中富集，单一保守的UGT 33酶（SfUGT 33 F28）专用于FAW肠道中的DIMBOA再葡糖基化。其编码基因的敲低以菌株特异性方式降低幼虫的性能。因此，这项研究表明，一个单一的UGT酶是负责解毒的主要玉米防御BXD在这种害虫。
The relationship between plants and insects is continuously evolving, and many insects rely on biochemical strategies to mitigate the effects of toxic chemicals in their food plants, allowing them to feed on well-defended plants. Spodoptera frugiperda, the fall armyworm (FAW), accepts a number of plants as hosts, and has particular success on plants of the Poaceae family such as maize, despite their benzoxazinoid (BXD) defenses. BXDs stored as inert glucosides are converted into toxic aglucones by plant glucosidases upon herbivory. DIMBOA, the main BXD aglucone released by maize leaves, can be stereoselectively re-glucosylated by UDP-glycosyltransferases (UGTs) in the insect gut, rendering it non-toxic. Here, we identify UGTs involved in BXD detoxification by FAW larvae and examine how RNAi-mediated manipulation of the larval glucosylation capacity toward the major maize BXD, DIMBOA, affects larval growth. Our findings highlight the involvement of members of two major UGT families, UGT33 and UGT40, in the glycosylation of BXDs. Most of the BXD excretion in the frass occurs in the form of glucosylated products. Furthermore, the DIMBOA-associated activity was enriched in the gut tissue, with a single conserved UGT33 enzyme (SfUGT33F28) being dedicated to DIMBOA re-glucosylation in the FAW gut. The knock-down of its encoding gene reduces larval performance in a strain-specific manner. This study thus reveals that a single UGT enzyme is responsible for detoxification of the major maize-defensive BXD in this pest insect.
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影响因子：
1.5
作者：
Dumas P;Legeai F;Lemaitre C;Scaon E;Orsucci M;Labadie K;Gimenez S;Clamens AL;Henri H;Vavre F;Aury JM;Fournier P;Kergoat GJ;d'Alençon E
通讯作者：
d'Alençon E
影响因子：
56.9
作者：
Frey, M;Chomet, P;Gierl, A
通讯作者：
Gierl, A
影响因子：
3.8
作者：
Ahn, Seung-Joon;Vogel, Heiko;Heckel, David G.
通讯作者：
Heckel, David G.
影响因子：
3.8
作者：
AHMAD, SA;HOPKINS, TL
通讯作者：
HOPKINS, TL
影响因子：
2.3
作者：
CAMPOS, F;ATKINSON, J;TIMMINS, G
通讯作者：
TIMMINS, G