Identification of Beef Heifers with Superior Uterine Capacity for Pregnancy.
Identification of Beef Heifers with Superior Uterine Capacity for Pregnancy.
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DOI：
10.1095/biolreprod.116.141390
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发表时间：
2016-08
影响因子：
3.6
通讯作者：
Spencer TE
中科院分区：
文献类型：
--
作者：
Geary TW;Burns GW;Moraes JG;Moss JI;Denicol AC;Dobbs KB;Ortega MS;Hansen PJ;Wehrman ME;Neibergs H;O'Neil E;Behura S;Spencer TE
不孕症和生育力低下是家畜和人类的主要问题，大多数胚胎丢失发生在妊娠第一个月，涉及妊娠识别和概念植入。子宫内膜中介导妊娠建立和成功的关键基因和生理途径尚不清楚。在研究一中，主要使用连续胚胎移植根据生育力对安格斯小母牛进行分类，以选择在妊娠损失方面具有内在差异的动物。在四轮中的每一轮中，在发情后第7天将一个体外产生的高质量胚胎移植到小母牛中，并在第28天和第42天通过超声波确定妊娠，然后终止。根据妊娠成功率，将小母牛分为高生育力 (HF)、低生育力 (SF) 或不生育力 (IF)。在研究二中，对生育力分类的小母牛进行重新同步，并用一头高生育力公牛的精液进行繁殖。从交配后第 0 天到第 36 天，每隔一天采集一次血样。第 28 天通过超声波确定妊娠率，HF 母牛的妊娠率 (70.4%) 高于低生育力小母牛 (36.8%；SF 和 IF)。在生育组中，发情后前 20 天内，非怀孕小母牛血清中的黄体酮浓度没有差异，怀孕小母牛也没有差异。在研究三中，在发情后第 7 天将单个体内产生的胚胎转移到生育力分类的小母牛中。第14天冲洗子宫以回收胚胎，并从同侧子宫角获取子宫内膜活检。小母牛组之间的胚胎恢复率以及概念长度和面积没有差异。对妊娠 HF、SF 和 IF 小母牛（每组 n = 5）第 14 天的子宫内膜活检组织进行 RNA 测序，并通过 EdgeR 稳健分析进行分析。与 SF 子宫内膜相比，HF 子宫内膜有 26 个差异表达基因（DEG），SF 与 IF 子宫内膜相比有 12 个差异表达基因（DEG），HF 和 IF 子宫内膜之间有 3 个差异表达基因（DEG）。一些 DEG 编码的蛋白质参与免疫反应并在 B 细胞中表达。结果表明，SF 和 IF 小母牛的着床前胚胎存活和生长至第 14 天并未受到影响。因此，在这些生育力分类的小母牛中观察到的妊娠成功能力差异在第 14 天和第 28 天之间很明显，此时妊娠识别信号传导以及概念伸长和着床必须发生才能建立妊娠。
Infertility and subfertility represent major problems in domestic animals and humans, and the majority of embryonic loss occurs during the first month of gestation that involves pregnancy recognition and conceptus implantation. The critical genes and physiological pathways in the endometrium that mediate pregnancy establishment and success are not well understood. In study one, predominantly Angus heifers were classified based on fertility using serial embryo transfer to select animals with intrinsic differences in pregnancy loss. In each of the four rounds, a single in vitro-produced, high-quality embryo was transferred into heifers on Day 7 postestrus and pregnancy was determined on Days 28 and 42 by ultrasound and then terminated. Heifers were classified based on pregnancy success as high fertile (HF), subfertile (SF), or infertile (IF). In study two, fertility-classified heifers were resynchronized and bred with semen from a single high-fertility bull. Blood samples were collected every other day from Days 0 to 36 postmating. Pregnancy rate was determined on Day 28 by ultrasound and was higher in HF (70.4%) than in heifers with low fertility (36.8%; SF and IF). Progesterone concentrations in serum during the first 20 days postestrus were not different in nonpregnant heifers and also not different in pregnant heifers among fertility groups. In study three, a single in vivo-produced embryo was transferred into fertility-classified heifers on Day 7 postestrus. The uteri were flushed on Day 14 to recover embryos, and endometrial biopsies were obtained from the ipsilateral uterine horn. Embryo recovery rate and conceptus length and area were not different among the heifer groups. RNA was sequenced from the Day 14 endometrial biopsies of pregnant HF, SF, and IF heifers (n = 5 per group) and analyzed by edgeR-robust analysis. There were 26 differentially expressed genes (DEGs) in the HF compared to SF endometrium, 12 DEGs for SF compared to IF endometrium, and three DEGs between the HF and IF endometrium. Several of the DEG-encoded proteins are involved in immune responses and are expressed in B cells. Results indicate that preimplantation conceptus survival and growth to Day 14 is not compromised in SF and IF heifers. Thus, the observed difference in capacity for pregnancy success in these fertility-classified heifers is manifest between Days 14 and 28 when pregnancy recognition signaling and conceptus elongation and implantation must occur for the establishment of pregnancy.
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DOI：
10.1530/jrf.0.0590205
发表时间：
1980-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
BETTERIDGE, KJ;EAGLESOME, MD;MITCHELL, D
通讯作者：
MITCHELL, D
DOI：
10.1530/jrf.0.0590463
发表时间：
1980-01-01
期刊：
JOURNAL OF REPRODUCTION AND FERTILITY
影响因子：
--
作者：
DISKIN, MG;SREENAN, JM
通讯作者：
SREENAN, JM
DOI：
10.1073/pnas.88.9.3952
发表时间：
1991-05-01
影响因子：
11.1
作者：
DIAMOND, G;ZASLOFF, M;BEVINS, CL
通讯作者：
BEVINS, CL
影响因子：
3.6
作者：
Gray, CA;Taylor, KM;Spencer, TE
通讯作者：
Spencer, TE
影响因子：
2.8
作者：
Berg, D. K.;van Leeuwen, J.;Pfeffer, P. L.
通讯作者：
Pfeffer, P. L.