Intracerebral hemorrhage leads to infiltration of several leukocyte populations with concomitant pathophysiological changes.
Intracerebral hemorrhage leads to infiltration of several leukocyte populations with concomitant pathophysiological changes.
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DOI：
10.1038/jcbfm.2008.114
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发表时间：
2009-01
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--
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中科院分区：
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脑出血(ICH)是卒中的一种亚型，具有较高的死亡率和发病率。免疫系统，特别是补体和细胞因子信号，与脑出血后的脑损伤有关。然而，与脑出血相关的细胞免疫学研究还不够深入。在这份报告中，我们使用流式细胞术对脑出血后1天和4天渗入脑内的免疫细胞群进行了定量分析。培养1d时，CD45hi GR-1+细胞较生理盐水对照组增加2.0倍(P≤0.05)，但未观察到其他细胞群的变化。与对照组相比，第4天脑出血小鼠的CD45hi细胞数增加了2.4倍，CD45hi GR-1细胞数增加了1.9倍，CD45hi GR-1+细胞数增加了3.4倍，最显著的是−+细胞数增加了1.7倍(所有组均为P≤0.05)。CD8+细胞和CD45loGR-1−细胞数未见明显变化(P分别为0.43和0.49)。因此，我们首次使用流式细胞术来分析脑出血后白细胞的渗透情况。我们对CD4T细胞浸润的发现是新的，这表明适应性免疫系统在脑出血的反应中发挥了作用。
Intracerebral hemorrhage (ICH) is a stroke subtype with high rates of mortality and morbidity. The immune system, particularly complement and cytokine signaling, has been implicated in brain injury after ICH. However, the cellular immunology associated with ICH has been understudied. In this report, we use flow cytometry to quantitatively profile immune cell populations that infiltrate the brain 1 and 4 days post-ICH. At 1 day CD45hi GR-1 + cells were increased 2.0-fold compared with saline controls (P≤0.05); however, we did not observe changes in any other cell populations analyzed. At 4 days ICH mice presented with a 2.4-fold increase in CD45hi cells, a 1.9-fold increase in CD45hi GR-1− cells, a 3.4-fold increase in CD45hi GR-1 + cells, and most notably, a 1.7-fold increase in CD4+ cells (P≤0.05 for all groups), compared with control mice. We did not observe changes in the numbers of CD8 + cells or CD45lo GR-1− cells (P = 0.43 and 0.49, respectively). Thus, we have shown the first use of flow cytometry to analyze leukocyte infiltration in response to ICH. Our finding of a CD4 T-cell infiltrate is novel and suggests a role for the adaptive immune system in the response to ICH.
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