Increased Dickkopf-1 expression is correlated with poisoning severity in carbon monoxide-poisoned humans and rats
Increased Dickkopf-1 expression is correlated with poisoning severity in carbon monoxide-poisoned humans and rats
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Dickkopf-1 表达增加与一氧化碳中毒的人和大鼠的中毒严重程度相关
DOI：
10.1080/08958378.2016.1198440
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发表时间：
2016-06
影响因子：
2.1
通讯作者：
Xing Jihong
中科院分区：
文献类型：
--
作者：
Pang Li;Dong Ning;Wang Dawei;Zhang Nan;Xing Jihong
摘要背景：一氧化碳(CO)中毒导致神经元损伤。Dickkopf-1(DKK-1)在一氧化碳中毒后的表达未见报道。目的：研究急性一氧化碳中毒大鼠和人的DKK-1的表达水平，并分析其与中毒严重程度的关系。材料和方法：检测急性一氧化碳中毒患者(n = 94)和健康对照组(n = 90)血清DKK-1水平。入院时，为每个患者确定中毒严重程度评分(PSS)。另外，36只雄性SD大鼠随机分为3组：(A)假手术组、(B)低CO组和(C)高CO组。于CO中毒后2 h检测大鼠海马区DKK-1的表达及组织病理学改变。结果：急性一氧化碳中毒患者血清DKK-1水平明显高于健康对照组。PSS评分较低的CO中毒患者血清DKK-1水平明显升高。CO中毒大鼠海马区DKK-1基因和蛋白表达显著上调。DKK-1水平与海马区组织损伤程度呈正相关。讨论：CO中毒后DKK-1在神经元中的表达可导致进一步的神经元损伤。急性一氧化碳中毒大鼠模型的严重程度与血清DKK-1水平升高及脑组织中DKK-1表达上调有关。结论：DKK-1在判断CO中毒的严重程度和损害程度方面具有潜在的实用价值。
Abstract Context: Carbon monoxide (CO) poisoning results in neuronal injury. The expression of Dickkopf-1 (DKK-1) has not been investigated previously after CO poisoning. Objective: The current study aimed to investigate the DKK-1 expression levels in humans and rats with acute CO poisoning and to analyze their correlation with poisoning severity. Materials and methods: We measured serum DKK-1 levels in patients with acute CO poisoning (n = 94) and in healthy controls (n = 90). On admission, a poisoning severity score (PSS) was determined for each patient. In addition, 36 male Sprague–Dawley rats were randomly assigned into three groups: (a) Sham group, (b) Low CO group and (c) High CO group. At 2 h after CO poisoning, DKK-1 expression and histopathological damage in the hippocampal tissues were measured. Results: Serum DKK-1 levels were significantly higher in the acute CO-poisoned patients, compared to the healthy controls. Serum DKK-1 levels were significantly higher in the CO-poisoned patients with a lower PSS. In rats, CO poisoning induced significant upregulation of the gene and protein expression of DKK-1 in hippocampal tissues. Moreover, there was a positive correlation between DKK-1 levels and the degree of damage in the hippocampal tissues. Discussion: DKK-1 induction in neurons after CO poisoning causes further neuronal injury. The severity of acute CO poisoning in rat models is associated with elevated serum DKK-1 levels and its upregulation in the brain tissue. Conclusion: DKK-1 appears to have potential utility in providing valuable information for determining the severity and damage of CO poisoning.
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DOI：
10.1016/j.ajem.2008.04.016
发表时间：
2009-09
期刊：
The American journal of emergency medicine
影响因子：
--
作者：
T. Yardan;Y. Çevik;O. Donderici;C. Kavalcı;F. Yilmaz;G. Yilmaz;K. Vural;Y. Yuzbasioglu;Y. Gunaydin;A. Sezer
通讯作者：
T. Yardan;Y. Çevik;O. Donderici;C. Kavalcı;F. Yilmaz;G. Yilmaz;K. Vural;Y. Yuzbasioglu;Y. Gunaydin;A. Sezer
DOI：
10.3109/15563659809028940
发表时间：
1998-01-01
期刊：
JOURNAL OF TOXICOLOGY-CLINICAL TOXICOLOGY
影响因子：
--
作者：
Persson, HE;Sjöberg, GK;de Garbino, JP
通讯作者：
de Garbino, JP
影响因子：
6.1
作者：
Scali, Carla;Caraci, Filippo;Caricasole, Andrea
通讯作者：
Caricasole, Andrea
影响因子：
1.8
作者：
Kashanian, Maryam;Shahali, Shadab
通讯作者：
Shahali, Shadab
DOI：
--
发表时间：
2004
期刊：
Nature reviews. Genetics
影响因子：
--
作者：
R. Moon;A. D. Kohn;G. D. De Ferrari;A. Kaykas
通讯作者：
R. Moon;A. D. Kohn;G. D. De Ferrari;A. Kaykas