Metagenomic analyses highlight the symbiotic association between the glacier stonefly Andiperla willinki and its bacterial gut community
Metagenomic analyses highlight the symbiotic association between the glacier stonefly Andiperla willinki and its bacterial gut community
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宏基因组分析强调了冰川石蝇 Andiperla willinki 与其肠道细菌群落之间的共生关系
DOI：
10.1111/1462-2920.14420
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发表时间：
2018
影响因子：
5.1
通讯作者：
Y.
中科院分区：
文献类型：
--
作者：
Murakami;T.;Segawam T.;Takeuchi;N.;Barcaza;G.;Lavarcam P.;Kohshima;S.;Hongoh;Y.
冰川石蝇Andiperla willinki是巴塔哥尼亚冰川上最大的后生动物。在这项研究中，我们分析了肠道微生物组的水生生物的16S rRNA基因扩增和宏基因组测序。肠道细菌群落一直由动物消化道的典型类群（如Dysgonomonadaceae和Lachnospiraceae）以及冰川环境中的土著类群（如Polaromonas）主导。有趣的是，在石肠中检测到的占主导地位的Polaromonasalyprotium类型几乎从未在冰川表面栖息地检测到。原位杂交分析表明，动物肠道典型的细菌谱系以共聚集的形式定殖于肠壁，而聚合物中不包括Polaromonas细胞。从宏基因组数据集重建了几种优势细菌谱系的基因组草案，并表明优势Dysgonomonadaceaebacterium能够降解来源于宿主摄取的食物（如藻类）的各种多糖，其他优势细菌谱系发酵多糖降解释放的多糖。我们的研究结果表明，肠道细菌宿主协会在冰川石有助于宿主营养以及在冰川环境中的物质循环。
The glacier stoneflyAndiperla willinkiis the largest metazoan inhabiting the Patagonian glaciers. In this study, we analysed the gut microbiome of the aquatic nymphs by 16S rRNA gene amplicon and metagenomic sequencing. The bacterial gut community was consistently dominated by taxa typical of animal digestive tracts, such asDysgonomonadaceaeandLachnospiraceae, as well as those generally indigenous to glacier environments, such asPolaromonas. Interestingly, the dominantPolaromonasphylotypes detected in the stonefly gut were almost never detected in the glacier surface habitat. Fluorescencein situhybridization analysis revealed that the bacterial lineages typical of animal guts colonized the gut wall in a co‐aggregated form, whilePolaromonascells were not included in the aggregates. Draft genomes of several dominant bacterial lineages were reconstructed from metagenomic datasets and indicated that the predominantDysgonomonadaceaebacterium is capable of degrading various polysaccharides derived from host‐ingested food, such as algae, and that other dominant bacterial lineages ferment saccharides liberated by the polysaccharide degradation. Our results suggest that the gut bacteria–host association in the glacier stonefly contributes to host nutrition as well as material cycles in the glacier environment.
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影响因子：
5.2
作者：
Terashima M;Umezawa K;Mori S;Kojima H;Fukui M
通讯作者：
Fukui M
南极科利菌（Klebsormidiales，绿藻门）细胞壁的超微结构、化学成分和生物合成
DOI：
10.1080/09670260010001735931
发表时间：
2000
期刊：
影响因子：
--
作者：
G. Piro;M. Lenucci;G. Dalessandro;N. La Rocca;N. Rascio;I. Moro;C. Andreoli
通讯作者：
C. Andreoli
影响因子：
48
作者：
Langmead, Ben;Salzberg, Steven L.
通讯作者：
Salzberg, Steven L.
影响因子：
3.7
作者：
Green ER;Mecsas J
通讯作者：
Mecsas J
影响因子：
4.3
作者：
Borisov, Vitaliy B.;Gennis, Robert B.;Hemp, James;Verkhovsky, Michael I.
通讯作者：
Verkhovsky, Michael I.