Ptf1a is expressed transiently in all types of amacrine cells in the embryonic zebrafish retina.
Ptf1a is expressed transiently in all types of amacrine cells in the embryonic zebrafish retina.
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DOI：
10.1186/1749-8104-4-34
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发表时间：
2009-09-04
影响因子：
3.6
通讯作者：
Harris WA
中科院分区：
文献类型：
--
作者：
Jusuf PR;Harris WA
脊椎动物视网膜由五种主要类型的神经元组成：三种兴奋性（感光细胞，双极细胞和神经节细胞）和两个抑制性（水平和无肿瘤细胞）。转录因子PTF1A（胰腺转录因子1A）对于抑制性视网膜神经元的正常发育很重要。
使用转基因PTF1A：GFP报告基因和斑马鱼视网膜的原位杂交，我们表明PTF1A消息在在视网膜神经epithelium的尖端表面的末端分裂之后，在所有的无分支机构和水平细胞中都在所有无分和水平细胞中暂时表达。随着这些细胞迁移到最终的层流位置。然后，该消息关闭，但是我们可以在施用后120小时遵循稳定的PTF1A：GFP蛋白。在此胚胎时间点上，已经可以区分各种在解剖学和神经化学上不同的小节细胞的亚型。
PTF1A表达的时间表明，它参与了无链氨酸细胞分化的早期阶段或步骤，由于PTF1A：GFP的持久性，可以看到它们可以迅速多样化成大量亚型。这项工作为未来的研究奠定了阶段，研究了无长血性亚型的遗传规范。
The vertebrate retina is composed of five major types of neurons: three excitatory (photoreceptors, bipolar cells and ganglion cells) and two inhibitory (horizontal and amacrine cells). The transcription factor Ptf1a (pancreas transcription factor 1a) is important for the normal development of the inhibitory retinal neurons. Using a transgenic Ptf1a:GFP reporter and in situ hybridization in the zebrafish retina, we show that ptf1a message is transiently expressed in all amacrine and horizontal cells within hours after the terminal division of multipotent progenitors at the apical surface of the retinal neuroepithelium, and remains on as these cells migrate to their final laminar location. The message then shuts off, but we can follow the stable Ptf1a:GFP protein for up to 120 hours post-fertilization. A variety of anatomically and neurochemically distinct subtypes of amacrine cells can already be distinguished at this embryonic time point. The timing of Ptf1a expression suggests that it is involved in the very early stages or steps in the differentiation of amacrine cells, which, due to the perdurance of the Ptf1a:GFP, can be seen to rapidly diversify into a large number of subtypes. This work sets the stage for future studies looking at genetic specification of amacrine subtypes.
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