Crucial roles of the pentatricopeptide repeat protein SOAR1 in Arabidopsis response to drought, salt and cold stresses.
Crucial roles of the pentatricopeptide repeat protein SOAR1 in Arabidopsis response to drought, salt and cold stresses.
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DOI：
10.1007/s11103-015-0327-9
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发表时间：
2015-07
影响因子：
5.1
通讯作者：
Zhang, Da-Peng
中科院分区：
文献类型：
--
作者：
Jiang, Shang-Chuan;Mei, Chao;Liang, Shan;Yu, Yong-Tao;Lu, Kai;Wu, Zhen;Wang, Xiao-Fang;Zhang, Da-Peng
关键词：
虽然几个线粒体/叶绿体五肽重复（PPR）蛋白已被报道，以调节植物对非生物胁迫的反应，没有核定位的PPR蛋白已被发现在这些过程中发挥作用。在本实验中，我们提供的证据表明，一个胞质-核双本地化的PPR蛋白SOAR 1，功能负调节脱落酸（阿坝）信号在种子萌发和发芽后的生长，是一个至关重要的，积极的调节植物对非生物胁迫的反应。SOAR 1表达下调降低，但上调SOAR 1表达增强，ABA诱导的促进气孔关闭和抑制气孔开放的阿坝敏感性，和植物耐受多种，主要的非生物胁迫，包括干旱，高盐和低温。有趣且重要的是，SOAR 1过表达株系显示出很强的耐旱、耐盐和耐冷胁迫的能力，在能够在海水中潜在萌发的种子的萌发和萌发后生长中具有令人惊讶的高耐盐胁迫性，而没有观察到对植物生长和发育的负面影响。因此，SOAR 1基因可能有助于通过转基因操作来改良作物，以提高作物在胁迫条件下的生产力。进一步的实验数据表明，SOAR 1可能至少部分地通过整合ABA依赖性和非依赖性信号传导途径来调节植物胁迫反应，这不同于ABI 2/ABI 1 2C型蛋白磷酸酶介导的阿坝信号传导。这些发现有助于理解高度复杂的胁迫和阿坝信号网络。本文的在线版本（doi：10.1007/s11103-015-0327-9）包含补充材料，可供授权用户使用。
Whereas several mitochondrial/chloroplast pentatricopeptide repeat (PPR) proteins have been reported to regulate plant responses to abiotic stresses, no nucleus-localized PPR protein has been found to play role in these processes. In the present experiment, we provide evidence that a cytosol-nucleus dual-localized PPR protein SOAR1, functioning to negatively regulate abscisic acid (ABA) signaling in seed germination and postgermination growth, is a crucial, positive regulator of plant response to abiotic stresses. Downregulation of SOAR1 expression reduces, but upregulation of SOAR1 expression enhances, ABA sensitivity in ABA-induced promotion of stomatal closure and inhibition of stomatal opening, and plant tolerance to multiple, major abiotic stresses including drought, high salinity and low temperature. Interestingly and importantly, the SOAR1-overexpression lines display strong abilities to tolerate drought, salt and cold stresses, with surprisingly high resistance to salt stress in germination and postgermination growth of seeds that are able to potentially germinate in seawater, while no negative effect on plant growth and development was observed. So, the SOAR1 gene is likely useful for improvement of crops by transgenic manipulation to enhance crop productivity in stressful conditions. Further experimental data suggest that SOAR1 likely regulates plant stress responses at least partly by integrating ABA-dependent and independent signaling pathways, which is different from the ABI2/ABI1 type 2C protein phosphatase-mediated ABA signaling. These findings help to understand highly complicated stress and ABA signalling network. The online version of this article (doi:10.1007/s11103-015-0327-9) contains supplementary material, which is available to authorized users.
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影响因子：
7.4
作者：
Gilmour, SJ;Sebolt, AM;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
7.3
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影响因子：
5.1
作者：
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通讯作者：
Zhang, Da-Peng
影响因子：
7.2
作者：
Gilmour, SJ;Zarka, DG;Thomashow, MF
通讯作者：
Thomashow, MF
影响因子：
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作者：
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Zhu, JK