Vitamin C distribution and retention in the mouse brain.
Vitamin C distribution and retention in the mouse brain.
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DOI：
10.1016/j.brainres.2010.05.090
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发表时间：
2010-08-12
期刊：
影响因子：
2.9
通讯作者：
May, James M.
中科院分区：
文献类型：
--
作者：
Harrison, Fiona E.;Green, Roslyn J.;Dawes, Sean M.;May, James M.
维生素C（VC）是大脑中至关重要的抗氧化剂。为了评估不同的大脑区域是否对VC消耗引起的氧化应激敏感性不同，我们使用了古洛糖酸内酯氧化酶（gulo）敲除小鼠。这种小鼠和人类一样，不能合成VC，因此其组织VC水平可以通过饮食VC摄入量而变化。给Gulo基因敲除小鼠喂食含有标准（0.33 g/L）、低（0.033 g/L）或零（0 g/L）VC补充水平的饮用水。4周后，处死小鼠，取出不同脑区用于测定VC和丙二醛（脂质过氧化的标志物）。与年龄匹配的野生型对照组相比，小脑、嗅球和额叶皮质的VC含量最高，而脑桥和脊髓的VC含量最低。然而，在未接受VC的小鼠中，面积差异不再显著，因为所有值均趋向于零。随着VC补充量的减少，皮质中丙二醛含量增加。在小脑或脑桥中没有观察到相同的变化，这表明皮质更容易受到低VC的氧化损伤。这些结果表明，与其他脑区相比，皮层对低VC诱导的氧化应激的敏感性增强。
Vitamin C (VC) is a crucial antioxidant in the brain. To assess whether different brain regions vary in their sensitivity to oxidative stress induced by VC depletion, we used the gulonolactone oxidase (gulo) knockout mouse. This mouse, like humans, cannot synthesize VC and thus its tissue VC levels can be varied by dietary VC intake. Gulo knockout mice were fed drinking water containing standard (0.33 g/L), low (0.033 g/L) or zero (0 g/L) VC supplementation levels. After 4 weeks, mice were sacrificed and different brain regions removed for assay of VC and malondialdehyde, a marker of lipid peroxidation. Compared to age-matched wild-type controls, the cerebellum, olfactory bulbs and frontal cortex had the highest VC content, whereas the pons and spinal chord had the lowest. However, in mice that did not receive VC, area differences were no longer significant as all values trended towards zero. Malondialdehyde increased in the cortex as VC supplementation was decreased. The same changes were not observed in the cerebellum or pons, suggesting that cortex is more susceptible to oxidative damage from low VC. These results suggest enhanced susceptibility of the cortex to oxidative stress induced by low VC compared to other brain regions.
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影响因子：
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作者：
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通讯作者：
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