A novel statistical methodology for quantifying the spatial arrangements of axons in peripheral nerves.
A novel statistical methodology for quantifying the spatial arrangements of axons in peripheral nerves.
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DOI：
10.3389/fnins.2023.1072779
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发表时间：
2023
影响因子：
4.3
通讯作者：
Rajwa, Bartek
中科院分区：
文献类型：
--
作者：
Shemonti, Abida Sanjana;Plebani, Emanuele;Biscola, Natalia P.;Jaffey, Deborah M.;Havton, Leif A.;Keast, Janet R.;Pothen, Alex;Dundar, M. Murat;Powley, Terry L.;Rajwa, Bartek
关键词：
为了更好地了解周围神经的功能和开发神经调节工具和策略，需要对周围神经的神经解剖学有一个全面的了解。在生物物理建模中，通常认为周围神经中有髓和无髓轴突的复杂空间排列是随机的，但实际上轴突的组织是不均匀和各向异性的。目前的定量神经解剖学方法是根据轴突的数量和轴突的面积、直径等形态特征来分析周围神经。在本研究中，我们采用空间统计和点过程模型来描述轴突的空间排列和Sinkhorn距离，以计算各种迷走神经和骨盆神经横断面中这些排列之间的相似性（根据一阶和二阶统计）。我们利用高分辨率透射电子显微镜（TEM）图像，这些图像使用基于高度改进的U-Net架构的定制高通量深度学习系统进行分割。我们的研究结果展示了一种新颖和创新的方法来量化空间点模式之间的相似性，使用从最优运输问题的解决方案中得到的度量。我们还提出了一种可用于周围神经结构定量分析的通用管道。我们的数据显示了男性和女性样本之间的差异以及骨盆和腹部迷走神经之间的相似性。
A thorough understanding of the neuroanatomy of peripheral nerves is required for a better insight into their function and the development of neuromodulation tools and strategies. In biophysical modeling, it is commonly assumed that the complex spatial arrangement of myelinated and unmyelinated axons in peripheral nerves is random, however, in reality the axonal organization is inhomogeneous and anisotropic. Present quantitative neuroanatomy methods analyze peripheral nerves in terms of the number of axons and the morphometric characteristics of the axons, such as area and diameter. In this study, we employed spatial statistics and point process models to describe the spatial arrangement of axons and Sinkhorn distances to compute the similarities between these arrangements (in terms of first- and second-order statistics) in various vagus and pelvic nerve cross-sections. We utilized high-resolution transmission electron microscopy (TEM) images that have been segmented using a custom-built high-throughput deep learning system based on a highly modified U-Net architecture. Our findings show a novel and innovative approach to quantifying similarities between spatial point patterns using metrics derived from the solution to the optimal transport problem. We also present a generalizable pipeline for quantitative analysis of peripheral nerve architecture. Our data demonstrate differences between male- and female-originating samples and similarities between the pelvic and abdominal vagus nerves.
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影响因子：
4.7
作者：
Breit S;Kupferberg A;Rogler G;Hasler G
通讯作者：
Hasler G
影响因子：
2.5
作者：
HULSEBOSCH, CE;COGGESHALL, RE
通讯作者：
COGGESHALL, RE
影响因子：
--
作者：
BJAALIE, JG;DIGGLE, PJ;BRODAL, P
通讯作者：
BRODAL, P
影响因子：
--
作者：
HOFFMAN, HH;SCHNITZLEIN, HN
通讯作者：
SCHNITZLEIN, HN
DOI：
10.1023/b:visi.0000036836.66311.97
发表时间：
2004-12-01
影响因子：
19.5
作者：
Haker, S;Zhu, L;Angenent, S
通讯作者：
Angenent, S