Bacterial variation in the oral microbiota in multiple sclerosis patients.
Bacterial variation in the oral microbiota in multiple sclerosis patients.
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DOI：
10.1371/journal.pone.0260384
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发表时间：
2021
期刊：
影响因子：
3.7
通讯作者：
Abiri R
中科院分区：
文献类型：
--
作者：
Zangeneh Z;Abdi-Ali A;Khamooshian K;Alvandi A;Abiri R
口腔中的微生物被称为口腔微生物群，而微生物组由宿主体内微生物的总基因组含量组成。宿主和微生物之间的相互作用对于神经系统发育和神经疾病（例如自闭症、阿尔茨海默病、帕金森病和多发性硬化症 (MS)）非常重要。细菌感染作为多发性硬化症发病机制中的环境因素，可促进辅助性T细胞17（Th17）增加，并增强促炎细胞因子如白细胞介素21（IL-21）、IL-17和IL -22的产生。口腔微生物群由多种可培养和不可培养的细菌物种组成。变性梯度凝胶电泳（DGGE）是鉴定不可培养细菌的可接受的方法。在这项研究中，我们比较了多发性硬化症和健康人口腔中的细菌种群多样性。 2019 年 10 月至 2019 年 3 月，样本在克曼沙医科大学多发性硬化症患者中心采集。总共 30 个样本取自 MS 患者，另外 30 个样本取自健康人。获得纯培养物后，使用表型测试来鉴定细菌。从 1 mL 唾液中提取 DNA，用引物产生的 PCR 产物在聚丙烯酰胺凝胶上进行电泳。 MS 组中葡萄球菌属、放线菌属、梭杆菌属、拟杆菌属、卟啉单胞菌属、普雷沃氏菌属、韦荣球菌属、丙酸杆菌属和登录号为 MW880919-25、JQ477416.1、KF074888.1 的不可培养细菌和其他几种不可培养菌株在 MS 组中显着更丰富，而乳酸杆菌和根据逻辑回归方法，消化链球菌在正常健康组中更为普遍。口腔微生物可能会减轻或加剧影响多发性硬化症发病机制的炎症状况。可以假设控制口腔感染可能会导致多发性硬化症疾病进展的减缓。
Microorganisms in oral cavity are called oral microbiota, while microbiome consists of total genome content of microorganisms in a host. Interaction between host and microorganisms is important in nervous system development and nervous diseases such as Autism, Alzheimer, Parkinson and Multiple Sclerosis (MS). Bacterial infections, as an environmental factor in MS pathogenesis play role in T helper 17(Th17) increase and it enhancing the production of pro-inflammatory cytokines such as Interlukin-21(IL-21), IL-17 and IL -22. Oral microbiota consists diverse populations of cultivable and uncultivable bacterial species. Denaturing gradient gel electrophoresis (DGGE) is an acceptable method for identification of uncultivable bacteria. In this study, we compared the bacterial population diversity in the oral cavity between MS and healthy people. From October to March 2019, samples were taken at Kermanshah University of Medical Sciences’ MS patients center. A total of 30 samples were taken from MS patients and another 30 samples were taken from healthy people. Phenotypic tests were used to identify bacteria after pure cultures were obtained. DNA was extracted from 1 mL of saliva, and PCR products produced with primers were electrophoresed on polyacrylamide gels. The genera Staphylococcus, Actinomyces, Fusobacterium, Bacteroides, Porphyromonas, Prevotella, Veillonella, Propionibacterium and uncultivable bacteria with accession number MW880919-25, JQ477416.1, KF074888.1 and several other un-culturable strains were significantly more abundant in the MS group while Lactobacillus and Peptostreptococcus were more prevalent in the normal healthy group according to logistic regression method. Oral micro-organisms may alleviate or exacerbate inflammatory condition which impact MS disease pathogenesis. It may be assumed that controlling oral infections may result in reduction of MS disease progression.
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影响因子：
12.2
作者：
Mestre L;Carrillo-Salinas FJ;Feliú A;Mecha M;Alonso G;Espejo C;Calvo-Barreiro L;Luque-García JL;Estevez H;Villar LM;Guaza C
通讯作者：
Guaza C
影响因子：
5.8
作者：
通讯作者：
--
DOI：
10.15171/bi.2017.22
发表时间：
2017
期刊：
BioImpacts : BI
影响因子：
--
作者：
Asoudeh-Fard A;Barzegari A;Dehnad A;Bastani S;Golchin A;Omidi Y
通讯作者：
Omidi Y
影响因子：
5.2
作者：
Lukiw WJ
通讯作者：
Lukiw WJ
影响因子：
16.6
作者：
Jangi S;Gandhi R;Cox LM;Li N;von Glehn F;Yan R;Patel B;Mazzola MA;Liu S;Glanz BL;Cook S;Tankou S;Stuart F;Melo K;Nejad P;Smith K;Topçuolu BD;Holden J;Kivisäkk P;Chitnis T;De Jager PL;Quintana FJ;Gerber GK;Bry L;Weiner HL
通讯作者：
Weiner HL