Fluorescence and diffuse reflectance provide similar accuracy in recovering fluorophore concentration at short source-detector separations.
Fluorescence and diffuse reflectance provide similar accuracy in recovering fluorophore concentration at short source-detector separations.
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DOI：
10.1080/09500340.2022.2074159
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发表时间：
2022
影响因子：
1.3
通讯作者：
中科院分区：
文献类型：
--
作者：
定量荧光光谱法需要相应的反射率测量来校正组织吸收和散射。然而，目前还不清楚荧光是否增加了校正所需的漫反射测量值。本研究的目的是比较荧光和漫反射光谱的准确性，在恢复的高消光荧光团，亚甲蓝（MB）的浓度，使用紧凑的光纤探头。利用光源-探测器间距为288-1300 μm的光纤探针对模拟人体组织的荧光和漫反射进行了测量。回收的荧光团浓度的平均误差为20.4%的荧光和15.0%的反射率，虽然这种差异并不显着（p=0.77）。两种方法返回的浓度与已知MB浓度相似（两种情况下p≥0.79）。相对于漫反射，高消光荧光团浓度的荧光定量没有显著提高准确度。研究者应考虑荧光测量对于给定应用是否必要。
Quantitative fluorescence spectroscopy requires corresponding reflectance measurements to correct for tissue absorption and scattering. However, it is unclear whether fluorescence adds value beyond the diffuse reflectance measurements necessary for correction. The goal of this study was to compare the accuracy of fluorescence and diffuse reflectance spectroscopy in recovering the concentration of a high-extinction fluorophore, methylene blue (MB), using a compact fiber-optic probe. Fluorescence and diffuse reflectance measurements of tissue simulating phantoms were made using a fiber-optic probe with source-detector separations of 288-1300 μm. Average error in recovered fluorophore concentration was 20.4% for fluorescence and 15.0% for reflectance, though this difference was not significant (p=0.77). Both methods returned concentrations that were similar to known MB concentrations (p≥0.79 in both cases). Fluorescence quantification of the concentration of a high extinction fluorophore did not significantly improve accuracy relative to diffuse reflectance. Investigators should consider whether fluorescence measurements are necessary for a given application.
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