Raman spectroscopic detection of Nickel impact on single Streptomyces cells – possible bioindicators for heavy metal contamination
Raman spectroscopic detection of Nickel impact on single Streptomyces cells – possible bioindicators for heavy metal contamination
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拉曼光谱检测镍对单个链霉菌细胞的影响——重金属污染的可能生物指示剂
DOI:
10.1002/jrs.3126
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发表时间:
2012
影响因子:
2.5
通讯作者:
Popp J
中科院分区:
文献类型:
--
作者:
Walter A;Kuhri S;Reinicke M;Bocklitz T;Schumacher W;Rösch P;Merten D;Büchel G;Kothe E;Popp J
Heavy metal contamination of soil has an immense impact on the surrounding environment, such as the ground water, and hence, has become an important issue within bioremediation. Therefore, heavy metal contamination has to be determined preferably cost‐efficiently, rapidly, and reliably. Here, soil bacteria of the genusStreptomycesare used as bioindicators for heavy metal contamination investigated via micro‐Raman spectroscopy. A single cell approach is studied to avoid time‐consuming culturing and plate counting. Bacteria ofStreptomyces galilaeuswere incubated in Ni2+enriched media and single cell spectra were recorded. Supervised statistics linear discriminant analysis was used to evaluate the influence of the culture age and the anion on bacterial cells, which has been determined to be minor compared with the spectral impact of Ni2+. The identification of the Raman spectra according to different Ni2+concentration ranges is accomplished with a prediction accuracy of about 88%. Therefore, we conclude thatStreptomycescan be used as a bioindicator to predict Ni2+concentrations in the micromolar range. Copyright © 2011 John Wiley & Sons, Ltd.
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DOI:
--
发表时间:
1976
期刊:
Zeitschrift für allgemeine Mikrobiologie
影响因子:
--
作者:
E. Schuhmann†;F. Bergter
通讯作者:
F. Bergter
影响因子:
2.9
作者:
Gaus, K.;Roesch, P.;Popp, J.
通讯作者:
Popp, J.
DOI:
--
发表时间:
1996
期刊:
影响因子:
--
作者:
V. D. Mei;D. Naumann;H. Busscher
通讯作者:
H. Busscher
DOI:
--
发表时间:
1976
期刊:
影响因子:
--
作者:
E. Schuhmann;F. Bergter
通讯作者:
F. Bergter
影响因子:
3.8
作者:
S. Vaidyanathan;S. White;L. Harvey;B. McNeil
通讯作者:
B. McNeil