Raman spectroscopic detection of Nickel impact on single Streptomyces cells – possible bioindicators for heavy metal contamination

Raman spectroscopic detection of Nickel impact on single Streptomyces cells – possible bioindicators for heavy metal contamination
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拉曼光谱检测镍对单个链霉菌细胞的影响——重金属污染的可能生物指示剂

DOI:
10.1002/jrs.3126
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发表时间:
2012
影响因子:
2.5
通讯作者:
Popp J
Popp J
中科院分区:
化学3区
文献类型:
--
作者:
Walter A;Kuhri S;Reinicke M;Bocklitz T;Schumacher W;Rösch P;Merten D;Büchel G;Kothe E;Popp J

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土壤重金属污染对地下水等周围环境影响巨大,已成为生物修复中的一个重要问题。因此,必须最好以经济高效、快速和可靠的方式确定重金属污染。本文以土壤中链霉菌属细菌作为重金属污染的生物指示剂,通过显微拉曼光谱进行研究。研究了一种单细胞方法,以避免耗时的培养和平板计数。用富含Ni2+的培养液培养链霉菌,记录其单细胞光谱。用监督统计线性判别分析评价了培养年龄和阴离子对细菌细胞的影响,与Ni2+的光谱影响相比,这一影响是次要的。根据不同的Ni2+浓度范围对拉曼光谱进行了识别,预测准确率约为88%。因此,我们得出结论,链霉菌可以作为一种生物指示剂来预测微摩尔范围内的镍离子浓度。版权所有©2011 John Wiley&Sons,Ltd.
Heavy metal contamination of soil has an immense impact on the surrounding environment, such as the ground water, and hence, has become an important issue within bioremediation. Therefore, heavy metal contamination has to be determined preferably cost‐efficiently, rapidly, and reliably. Here, soil bacteria of the genusStreptomycesare used as bioindicators for heavy metal contamination investigated via micro‐Raman spectroscopy. A single cell approach is studied to avoid time‐consuming culturing and plate counting. Bacteria ofStreptomyces galilaeuswere incubated in Ni2+enriched media and single cell spectra were recorded. Supervised statistics linear discriminant analysis was used to evaluate the influence of the culture age and the anion on bacterial cells, which has been determined to be minor compared with the spectral impact of Ni2+. The identification of the Raman spectra according to different Ni2+concentration ranges is accomplished with a prediction accuracy of about 88%. Therefore, we conclude thatStreptomycescan be used as a bioindicator to predict Ni2+concentrations in the micromolar range. Copyright © 2011 John Wiley & Sons, Ltd.
吸水链霉菌生长动力学的显微研究。
DOI: --
发表时间: 1976
期刊: Zeitschrift für allgemeine Mikrobiologie
影响因子: --
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DOI: 10.1002/bip.20448
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Gaus, K.;Roesch, P.;Popp, J.
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通过 FT-IR 对不同生长培养基上生长的轻链球菌菌株进行分组
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DOI: --
发表时间: 1976
期刊:
影响因子: --
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DOI: --
发表时间: 2003
影响因子: 3.8
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