Protective effects of rutin against potassium bromate induced nephrotoxicity in rats.
Protective effects of rutin against potassium bromate induced nephrotoxicity in rats.
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DOI：
10.1186/1472-6882-12-204
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发表时间：
2012-11-01
影响因子：
--
通讯作者：
Sahreen S
中科院分区：
文献类型：
--
作者：
Khan RA;Khan MR;Sahreen S
本文研究了多酚类黄酮类化合物大黄素对KBrO_3致大鼠肾损伤的保护作用。I组为对照组（未处理），II组为生理盐水0.5 ml/kg bw（0.9%NaCl），III组为KBrO 3（20 mg/kg bw）灌胃，每周2次，共4周。第VI组（50 mg/kg bw）和第V组（70 mg/kg bw）沿着KBrO 3（20 mg/kg bw）给药，而第VI组仅给予芦丁（70 mg/kg bw），每周两次，持续四周。通过测定大鼠尿、血清生化指标、各种抗氧化酶、DNA含量及肾脏组织病理学改变，观察芦丁对KBrO_3所致大鼠肾毒性的保护作用。尿红细胞、尿白细胞、尿比重、尿素、肌酐、尿胆素原升高（P<0.01），肌酐清除率降低（P<0.01）。血清蛋白质、白蛋白、球蛋白、亚硝酸盐、肌酐和血尿素氮（BUN）水平显著升高（P<0.01）。确定肾组织中显著的组织病理学病变、升高的DNA片段化和AgNORs计数。抗氧化酶的活性; KBrO 3处理后，肾组织过氧化氢酶、超氧化物歧化酶、谷胱甘肽过氧化物酶、谷胱甘肽-S-转移酶、谷胱甘肽还原酶和还原型谷胱甘肽含量降低（P <0.01），硫代巴比妥酸反应物质增加（P<0.01）。DNA梯状条带分析与DNA片段化分析密切相关。KBrO_3处理组肾组织端粒酶活性呈阳性。芦丁治疗有效地改善了大鼠研究参数的变化。大鼠单独给予Ryno后，所研究的任何参数均未出现任何显著变化。这些结果表明，芦丁作为一种抗氧化剂，在体内清除活性氧，这有助于防止大鼠氧化性肾损伤与KBrO 3处理。
Rutin, a polyphenolic flavonoid, was investigated for its protective effects against the KBrO3 induced renal injuries in rat. Group I was control (untreated), group II was given saline 0.5 ml/kg bw (0.9% NaCl), group III was administered KBrO3 (20 mg/kg bw) intragastric twice a week for four weeks. Rutin was administered to group VI (50 mg/kg bw) and Group V (70 mg/kg bw) along with KBrO3 (20 mg/kg bw) while group VI was given rutin (70 mg/kg bw) alone twice a week for four weeks. Protective effects of rutin on KBrO3-induced nephrotoxicity in rats were determined for biochemical parameter of urine, and serum, various antioxidant enzymes, DNA and histopathological damages in kidneys. The level of urinary red blood cells, leucocytes count, specific gravity, urea, creatinine and urobilinogen was increased (P<0.01) whereas creatinine clearance was reduced. Serum level of protein, albumin, globulin, nitrite, creatinine and blood urea nitrogen (BUN) was significantly increased (P<0.01) by KBrO3. Marked histopathological lesions, elevated DNA fragmentation and AgNORs count in renal tissues was determined. Activity of antioxidant enzymes; catalase, superoxide dismutase, glutathione peroxidase, glutathione-S-transferase, glutathione reductase, and reduced glutathione contents were decreased (P<0.01) while thiobarbituric acid reactive substances were increased (P<0.01) with KBrO3 treatment in kidneys. DNA ladder assay was intimately related with the DNA fragmentation assay. Telomerase activity was found positive in the KBrO3 treated kidneys. Treatment with rutin effectively ameliorated the alterations in the studied parameters of rat. Rutin administration alone to rats did not exhibit any significant change in any of the parameters studied. These results suggest that rutin works as an antioxidant in vivo by scavenging reactive oxygen species and this serves to prevent oxidative renal damage in rat treated with KBrO3.
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