Reduced beta cell number rather than size is a major contributor to beta cell loss in type 2 diabetes.
Reduced beta cell number rather than size is a major contributor to beta cell loss in type 2 diabetes.
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DOI：
10.1007/s00125-021-05467-7
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发表时间：
2021-08
期刊：
影响因子：
8.2
通讯作者：
Itoh H
中科院分区：
文献类型：
--
作者：
Sasaki H;Saisho Y;Inaishi J;Watanabe Y;Tsuchiya T;Makio M;Sato M;Nishikawa M;Kitago M;Yamada T;Itoh H
2型糖尿病的特征是β细胞团(BCM)减少。然而，目前还不确定2型糖尿病患者BCM的减少是由于β细胞的尺寸或数量减少所致。我们的目的是检验胰岛细胞的大小和数量对患有和不患有2型糖尿病的人类胰岛形态的影响。胰腺标本取自行胰腺切除术的日本成年2型糖尿病患者(n = 26例)和非2型糖尿病患者(n = 38例)。使用胰岛素染色的胰腺组织，我们根据β细胞直径估计了β细胞的大小。胰岛细胞数量是由胰岛细胞面积分数和胰腺体积除以胰岛细胞大小的乘积来估算的。检测胰岛形态和代谢状态与胰岛细胞大小和数量的关系。与非糖尿病患者相比，2型糖尿病患者的β细胞大小(548.7 ± 58.5vs 606.7 ± 65.0μm~3，p <； 0.01)和数量(5.10 × 108 ± 2.35 × 108 vs 8.16 × 108 ± 4.27 × 108，p <； 0.01)均减小，且β细胞数量的相对减少(37%)大于β细胞大小(10%)。2型糖尿病患者和非2型糖尿病患者的β细胞数量与骨髓基质细胞数呈正相关(r = 分别为0.97和0.98，均为p 和 )，与糖化血红蛋白呈负相关(r = −分别为0.45和p 和 均为0.01)。在患有2型糖尿病的参与者中，β细胞的大小和数量都减少了，其中β细胞数量的相对减少更大。β细胞数量的减少似乎是2型糖尿病患者BCM减少的主要原因。本文的在线版本(10.1007/s00125-021-021-7)包含经过同行评审但未经编辑的补充材料。
Type 2 diabetes is characterised by reduced beta cell mass (BCM). However, it remains uncertain whether the reduction in BCM in type 2 diabetes is due to a decrease in size or number of beta cells. Our aim was to examine the impact of beta cell size and number on islet morphology in humans with and without type 2 diabetes. Pancreas samples were obtained from 64 Japanese adults with (n = 26) and without (n = 38) type 2 diabetes who underwent pancreatectomy. Using pancreatic tissues stained for insulin, we estimated beta cell size based on beta cell diameter. Beta cell number was estimated from the product of fractional beta cell area and pancreas volume divided by beta cell size. The associations of beta cell size and number with islet morphology and metabolic status were examined. Both beta cell size (548.7 ± 58.5 vs 606.7 ± 65.0 μm3, p < 0.01) and number (5.10 × 108 ± 2.35 × 108 vs 8.16 × 108 ± 4.27 × 108, p < 0.01) were decreased in participants with type 2 diabetes compared with those without diabetes, with the relative reduction in beta cell number (37%) being greater than for beta cell size (10%). Beta cell number but not size was positively correlated with BCM in participants with and without type 2 diabetes (r = 0.97 and r = 0.98, both p < 0.01) and negatively correlated with HbA1c (r = −0.45, p < 0.01). Both beta cell size and number were reduced in participants with type 2 diabetes, with the relative reduction in beta cell number being greater. Decrease in beta cell number appears to be a major contributor to reduced BCM in type 2 diabetes. The online version of this article (10.1007/s00125-021-05467-7) contains peer-reviewed but unedited supplementary material.
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影响因子：
7.7
作者：
Mezza T;Muscogiuri G;Sorice GP;Clemente G;Hu J;Pontecorvi A;Holst JJ;Giaccari A;Kulkarni RN
通讯作者：
Kulkarni RN
影响因子：
7.7
作者：
Sato, Seiji;Saisho, Yoshifumi;Itoh, Hiroshi
通讯作者：
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影响因子：
8.2
作者：
Sakuraba, H;Mizukami, H;Yagihashi, S
通讯作者：
Yagihashi, S
影响因子：
3.7
作者：
Hunter CS;Stein RW
通讯作者：
Stein RW
影响因子：
7.7
作者：
Butler, AE;Janson, J;Butler, PC
通讯作者：
Butler, PC