Isolation and characterization of human dental pulp derived stem cells by using media containing low human serum percentage as clinical grade substitutes for bovine serum.
Isolation and characterization of human dental pulp derived stem cells by using media containing low human serum percentage as clinical grade substitutes for bovine serum.
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通过使用含有低人类血清百分比的培养基作为牛血清的临床级替代品，对人牙纸浆衍生的干细胞的分离和表征。
DOI：
10.1371/journal.pone.0048945
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Curcio F
中科院分区：
文献类型：
--
作者：
Ferro F;Spelat R;Beltrami AP;Cesselli D;Curcio F
成体干细胞已被提议作为胚胎干细胞的替代物，以研究体外多谱系分化并用于治疗。目前用于分离和扩增成体干细胞的培养基需要使用大量动物血清，但动物源性培养试剂由于存在真实的感染和严重免疫反应的可能性而引发了一些问题。由于这些原因，需要动物血清的临床级替代品。我们测试了牙髓干细胞（DPSC）的分离，增殖，形态，干细胞相关的标志物表达，和成骨细胞分化潜力的化学成分确定的培养基中含有低百分比的人血清，1.25%，相比，培养基中含有10%胎牛血清（FBS）。在我们的分离/增殖培养基中加入低HS百分比培养的DPSC在没有免疫选择方法的情况下获得，并且在干细胞标志物的表达中显示出高度一致性，以更高的速率增殖，并且相对于在10%FBS中培养的DPSC表现出相当的成骨潜能。在这项研究中，我们证明了一个化学成分确定的培养基添加低HS百分比，来自自体和异源来源，可以是一个有效的替代FBS的培养基，并应有助于成人干细胞的临床应用。
Adult stem cells have been proposed as an alternative to embryonic stem cells to study multilineage differentiation in vitro and to use in therapy. Current culture media for isolation and expansion of adult stem cells require the use of large amounts of animal sera, but animal-derived culture reagents give rise to some questions due to the real possibility of infections and severe immune reactions. For these reasons a clinical grade substitute to animal sera is needed. We tested the isolation, proliferation, morphology, stemness related marker expression, and osteoblastic differentiation potential of Dental Pulp Stem Cells (DPSC) in a chemically defined medium containing a low percentage of human serum, 1.25%, in comparison to a medium containing 10% Fetal Bovine Serum (FBS). DPSCs cultured in presence of our isolation/proliferation medium added with low HS percentage were obtained without immune-selection methods and showed high uniformity in the expression of stem cell markers, proliferated at higher rate, and demonstrated comparable osteoblastic potential with respect to DPSCs cultured in 10% FBS. In this study we demonstrated that a chemically defined medium added with low HS percentage, derived from autologous and heterologous sources, could be a valid substitute to FBS-containing media and should be helpful for adult stem cells clinical application.
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影响因子：
3.7
作者：
Mohamet L;Lea ML;Ward CM
通讯作者：
Ward CM
影响因子：
4.1
作者：
Ferro, Federico;Falini, Giuseppe;Curcio, Francesco
通讯作者：
Curcio, Francesco
影响因子：
6
作者：
Ferro, Federico;Spelat, Renza;Curcio, Francesco
通讯作者：
Curcio, Francesco
影响因子：
4.1
作者：
Denker, H-W
通讯作者：
Denker, H-W
DOI：
10.1073/pnas.91.19.9004
发表时间：
1994-09-13
影响因子：
11.1
作者：
CURCIO, F;AMBESIIMPIOMBATO, FS;COON, HG
通讯作者：
COON, HG