The blood flow in pancreatico-duodenal grafts in rats: inhibition of nitric oxide synthase preferentially decreases islet blood flow.
The blood flow in pancreatico-duodenal grafts in rats: inhibition of nitric oxide synthase preferentially decreases islet blood flow.
复制标题
大鼠胰十二指肠移植物的血流量：抑制一氧化氮合酶优先减少胰岛血流量。
DOI：
10.1016/0014-2999(94)00783-4
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发表时间：
1995
影响因子：
5
通讯作者：
L. Jansson
中科院分区：
文献类型：
--
作者：
A. M. Svensson;S. Sandler;L. Jansson
本研究将正常血糖近交系Wistar-Furth大鼠植入同种异体胰腺-十二指肠移植，即去神经胰腺。移植后2周，用微球技术测量大鼠完整的自体胰腺和移植胰腺的血流量。血流测量前10min静脉注射生理盐水、NG-硝基-L精氨酸(25 mg/kg体重)或硝普钠(10μg/kg体重)。注射NG-硝基-L-精氨酸可使自体胰腺和移植胰腺的平均动脉压升高，全胰腺血流量明显减少。在自体胰腺和移植胰腺中，NG-硝基-L-精氨酸对胰岛血流量的减少更为明显，约占全胰腺血流量的4%，而对照组为10%。硝普钠显著降低平均动脉压，但不影响任何腺体的胰腺或胰岛血流量。结论：抑制一氧化氮合酶导致胰岛血流量优先减少，在自体胰腺和移植胰腺中都是如此。这表明，影响胰岛血流的一氧化氮主要来自内皮细胞，而不是来自外部神经纤维。
In this study normoglycemic inbred Wistar-Furth rats were implanted with a syngeneic pancreatico-duodenal graft, i.e. a denervated pancreas. The blood flow to the intact native pancreas and to the transplanted gland was measured with a microsphere technique in anesthetized rats 2 weeks after transplantation. The animals were given an intravenous injection with saline alone, NG-nitro-l-arginine (25 mg/kg body weight) or sodium nitroprusside (10 μg/kg body weight) 10 min before blood flow measurements. Administration of NG-nitro-l-arginine increased mean arterial blood pressure and caused a pronounced decrease in whole pancreatic blood flow in both the native and transplanted gland. The islet blood flow was more markedly decreased by NG-nitro-l-arginine in both the native and transplanted pancreas, and constituted about 4% of whole pancreatic blood flow compared with 10% in the control animals. Sodium nitroprusside markedly decreased mean arterial blood pressure, but did not affect pancreatic or islet blood flow in any of the glands. It is concluded that inhibition of nitric oxide synthase causes a preferential decrease in islet blood flow both in the native pancreas and in the transplanted pancreas. This suggests that nitric oxide which affects islet blood flow is mainly endothelial-derived, and does not emanate from external nervous fibers.
DOI：
10.1152/ajpgi.1982.242.6.g596
发表时间：
1982
期刊：
The American journal of physiology
影响因子：
--
作者：
Kvietys,PR;McLendon,JM;Bulkley,GB;Perry,MA;Granger,DN
通讯作者：
Granger,DN
大鼠脑毛细血管内皮的原代培养。
DOI：
10.1007/bf02618147
发表时间：
1981
期刊：
In vitro
影响因子：
--
作者：
Bowman,PD;Betz,AL;Ar,D;Wolinsky,JS;Penney,JB;Shivers,RR;Goldstein,GW
通讯作者：
Goldstein,GW