Production of heparin-containing hydrogels for modulating cell responses.
Production of heparin-containing hydrogels for modulating cell responses.
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DOI：
10.1016/j.actbio.2008.12.004
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发表时间：
2009-03
影响因子：
9.7
通讯作者：
Kiick, Kristi L.
中科院分区：
文献类型：
--
作者：
Nie, Ting;Akins, Robert E., Jr.;Kiick, Kristi L.
成功的组织再生要求生物材料具有最佳的生物活性和机械性能。可原位交联的含肝素水凝胶被设计成含有可调节量的生物组分（例如肝素、精氨酸-甘氨酸-天冬氨酸（RGD））以及表现出受控的机械性质（例如剪切模量）。这些凝胶参数也可以被调整以提供蛋白质（如生长因子）的受控递送，用于调节细胞行为。将马来酰亚胺官能化的低分子量肝素（LWMH）与聚（乙二醇）（PEG）水凝胶缀合。通过振荡流变学实验评估的弹性剪切模量可以通过水凝胶中聚合物的浓度和PEG的端基官能度来调节。使用两种不同模量（2.8和0.4 kPa）的水凝胶研究二维细胞培养实验中人主动脉外膜成纤维细胞（AoAF）反应的差异。这些实验表明，AoAF显示出对具有较高模量的材料的改善的粘附性。通过与PEG或LMWH缀合而与水凝胶连接的具有RGD的水凝胶的细胞反应的评价表明，当生物活性配体通过与PEG而不是与LMWH的连接而化学连接时，对这些材料的细胞反应得到改善。这些结果突出了重要的设计考虑，在裁剪这些材料的心血管组织工程应用。
Successful tissue regeneration requires that biomaterials have optimal bioactivity and mechanical properties. Heparin-containing hydrogels that can be crosslinked in situ were designed to contain tunable amounts of biological components (e.g. heparin, arginine–glycine–aspartate (RGD)) as well as to exhibit controlled mechanical properties (e.g. shear modulus). These gel parameters can also be tuned to provide controlled delivery of proteins, such as growth factors, for regulating cellular behavior. Maleimide-functionalized low-molecular-weight heparin (LWMH) was conjugated to a poly(ethylene glycol) (PEG) hydrogel. The elastic shear modulus, as assessed via oscillatory rheology experiments, could be tuned by the concentration of polymer in the hydrogel, and by the end group functionality of PEG. Hydrogels of two different moduli (2.8 and 0.4 kPa) were used to study differences in the response of human aortic adventitial fibroblasts (AoAF) in two-dimensional cell culture experiments. These experiments indicated that the AoAFs show improved adhesion to materials with the higher modulus. Evaluation of cell responses to hydrogels with RGD linked to the hydrogels via conjugation to PEG or to LMWH indicated improved cellular responses to these materials when the bioactive ligands were chemically attached through linkage to the PEG rather than to the LMWH. These results highlight important design considerations in the tailoring of these materials for cardiovascular tissue engineering applications.
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影响因子：
64.5
作者：
OBARA, M;KANG, MS;YAMADA, KM
通讯作者：
YAMADA, KM
影响因子：
--
作者：
Da Silva, MS;Horton, JA;Sobel, M
通讯作者：
Sobel, M
DOI：
10.1073/pnas.0605960103
发表时间：
2006-12-05
影响因子：
11.1
作者：
Kong, Hyun Joon;Boontheekul, Tanyarut;Mooney, David J.
通讯作者：
Mooney, David J.
影响因子：
10.8
作者：
Nie, Ting;Baldwin, Aaron;Kiick, Kristi L.
通讯作者：
Kiick, Kristi L.
DOI：
10.1152/ajpheart.00478.2002
发表时间：
2003-01-01
影响因子：
4.8
作者：
Cai, WJ;Koltai, S;Schaper, J
通讯作者：
Schaper, J