Osmolarity influences chondrocyte repair after injury in human articular cartilage.
Osmolarity influences chondrocyte repair after injury in human articular cartilage.
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DOI：
10.1186/s13018-015-0158-z
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发表时间：
2015-01-28
影响因子：
2.6
通讯作者：
Sun T
中科院分区：
文献类型：
--
作者：
Huang Y;Zhang Y;Ding X;Liu S;Sun T
目的是确定冲洗液渗透压对关节软骨细胞存活和代谢状态的影响后，机械损伤。从接受全膝关节置换术治疗骨关节炎的患者中采集骨软骨组织外植体，然后切穿其全层以建立机械损伤模型。将软骨外植体在具有不同渗透压（180、280、380、580 mOsm/L）的冲洗溶液（盐水和平衡盐）中孵育2小时。用激光共聚焦显微镜定量细胞死亡百分比（100 ×死细胞数/死细胞和活细胞数）。采用末端脱氧核苷酸转移酶介导的dUTP缺口末端标记法（TUNEL）检测损伤软骨细胞凋亡指数。用分光光度计在530 nm处测定蛋白多糖洗脱液的含量，用实时荧光定量PCR法测定HIF-1α和II型胶原mRNA的表达量。机械损伤后，原位死亡的软骨细胞主要位于损伤软骨边缘的浅切线区。随着渗透压的增加，细胞死亡率逐渐降低，蛋白多糖洗脱量逐渐减少。不同渗透压下TUNEL法检测细胞凋亡指数差异无统计学意义（P = 0.158）。180 mOsm/L组软骨细胞HIF-1α和II型胶原mRNA表达量最低，380 mOsm/L组最高。与生理盐水组相比，平衡盐组细胞死亡率显著降低（P = 0.001），蛋白多糖洗脱量也显著降低（P = 0.045）。平衡盐组软骨细胞HIF-1α（P = 0.017）和II型胶原（P = 0.034）mRNA表达量显著高于生理盐水组。灌洗液的渗透压对机械损伤后软骨细胞的存活和代谢状态起着重要作用，软骨细胞的死亡不是由凋亡引起的。增加灌洗液的渗透压可能具有软骨保护作用，可减少软骨细胞死亡，减少对代谢和蛋白聚糖洗脱的抑制，最终防止软骨退化并促进整体修复。
The purpose was to determine the influence of irrigation solution osmolarity on articular chondrocytes survival and metabolic state following mechanical injury. Osteochondral explants were harvested from patients undergoing total knee arthroplasty for osteoarthritis and then cut through their full thickness to establish mechanical injury models. Cartilage explants were incubated in irrigation solutions (saline and balanced salt) with different osmolarities (180, 280, 380, 580 mOsm/L) for 2 h. The percentage of cell death (100 × number of dead cells/number of dead and live cells) was quantified with the laser confocal microscopy. The terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay was performed to detect apoptosis index of injured cartilage. The contents of proteoglycan elution were determined by spectrophotometer at 530 nm, and HIF-1α and type II collagen mRNA yields were quantified with real-time PCR. In situ dead chondrocytes were mainly localized to the superficial tangential region of injured cartilage edge after mechanical injury. The percentage of cell death was decreased, and proteoglycan elution was gradually reduced with the increasing of osmolarity. The apoptosis indices of TUNEL assay in different osmolarities had no significant difference (P = 0.158). HIF-1α and type II collagen mRNA yields were the least for chondrocytes exposed to 180 mOsm/L medium and were the greatest for chondrocytes exposed to 380 mOsm/L medium. Compared with the saline group, the cell death of superficial zone was significantly decreased (P = 0.001) and contents of proteoglycan elution were also significantly decreased (P = 0.045) in the balanced salt. HIF-1α (P = 0.017) and type II collagen (P = 0.034) mRNA yields in the chondrocytes exposed to the balanced salt were significantly more than the saline group. The osmolarity of irrigation solutions plays an important role in the survival and metabolic state of chondrocytes following mechanical injury, and the chondrocyte death is not caused by apoptosis. Increasing osmolarity of irrigation solutions may be chondroprotective with decreasing the chondrocyte death, reducing inhibition of metabolism and proteoglycan elution, ultimately preventing cartilage degeneration and promoting integrative repair.
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影响因子：
--
作者：
Phan, Mimi N.;Leddy, Holly A.;Votta, Bartholomew J.;Kumar, Sanjay;Levy, Dana S.;Lipshutz, David B.;Lee, Suk Hee;Liedtke, Wolfgang;Guilak, Farshid
通讯作者：
Guilak, Farshid
影响因子：
3.8
作者：
COMPER, WD;WILLIAMS, RPW
通讯作者：
WILLIAMS, RPW
DOI：
10.2106/00004623-200300002-00007
发表时间：
2003-01-01
影响因子：
5.3
作者：
Mainil-Varlet, P;Aigner, T;Stauffer, E
通讯作者：
Stauffer, E
DOI：
10.1016/j.bbrc.2012.05.015
发表时间：
2012-06-08
影响因子：
3.1
作者：
Bertram, Karri L.;Krawetz, Roman J.
通讯作者：
Krawetz, Roman J.
影响因子：
5.6
作者：
Bush, PG;Hall, AC
通讯作者：
Hall, AC