A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle.
A newly established bovine intestinal epithelial cell line is effective for in vitro screening of potential antiviral immunobiotic microorganisms for cattle.
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DOI：
10.1016/j.rvsc.2011.10.002
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发表时间：
2012-10
影响因子：
2.4
通讯作者：
Kitazawa H
中科院分区：
文献类型：
--
作者：
Chiba E;Villena J;Hosoya S;Takanashi N;Shimazu T;Aso H;Tohno M;Suda Y;Kawai Y;Saito T;Miyazawa K;He F;Kitazawa H
我们评估了牛肠上皮（BIE）细胞系是否可以作为有用的体外模型系统，用于研究牛肠上皮细胞（IEC）的抗病毒免疫反应以及初步筛选具有抗病毒保护能力的免疫微生物。免疫荧光分析显示，BIE 细胞中表达了 Toll 样受体 3 (TLR3)，实时定量 PCR 结果显示，这些细胞通过上调促炎细胞因子和 I 型干扰素来响应 Poly(I:C) 刺激。此外，我们证明 BIE 细胞可用于免疫生物乳酸菌菌株的初步筛选，这些菌株能够有益地调节牛 IEC 中由 TLR3 激活引发的抗病毒免疫反应。本研究中进行的 BIE 细胞的表征代表了朝着建立有价值的牛体外系统迈出的重要一步，该系统可用于开发牛宿主的免疫调节饲料。
We evaluated whether a bovine intestinal epithelial (BIE) cell line could serve as a useful in vitro model system for studying antiviral immune responses in bovine intestinal epithelial cells (IECs) and for the primary screening of immunobiotic microorganisms with antiviral protective capabilities. Immunofluorescent analyses revealed that toll-like receptor 3 (TLR3) was expressed in BIE cells, and the results of real-time quantitative PCR showed that these cells respond to stimulation with poly(I:C) by up-regulating pro-inflammatory cytokines and type I interferons. In addition, we demonstrated that BIE cells are useful for the primary screening of immunobiotic lactic acid bacteria strains which are able to beneficially modulate antiviral immune responses triggered by TLR3 activation in bovine IECs. The characterization of BIE cells performed in the present study represents an important step towards the establishment of a valuable bovine in vitro system that could be used for the development of immunomodulatory feed for bovine hosts.
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影响因子：
2.2
作者：
Liu, Fangning;Li, Guohua;Yuan, Lijuan
通讯作者：
Yuan, Lijuan
TLR3识别双链RNA诱导小鼠严重小肠损伤
影响因子：
4.4
作者：
Zhou, Rongbin;Wei, Haiming;Tian, Zhigang
通讯作者：
Tian, Zhigang
影响因子：
3.1
作者：
Cario, E;Podolsky, DK
通讯作者：
Podolsky, DK
DOI：
10.1016/j.bbrc.2005.03.017
发表时间：
2005-05-06
影响因子：
3.1
作者：
Tohno, M;Shimosato, T;Saito, T
通讯作者：
Saito, T
影响因子：
2
作者：
Tohno, Masanori;Shimosato, Takeshi;Kitazawa, Haruki
通讯作者：
Kitazawa, Haruki