Monosynaptic Retrograde Tracing From Prelimbic Neuron Subpopulations Projecting to Either Nucleus Accumbens Core or Rostromedial Tegmental Nucleus.
Monosynaptic Retrograde Tracing From Prelimbic Neuron Subpopulations Projecting to Either Nucleus Accumbens Core or Rostromedial Tegmental Nucleus.
复制标题
投射到伏隔核核或头内侧被盖核的前额叶神经元亚群的单突触逆行追踪。
DOI：
10.3389/fncir.2021.639733
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发表时间：
2021
影响因子：
3.5
通讯作者：
Smith RJ
中科院分区：
文献类型：
--
作者：
Cruz AM;Kim TH;Smith RJ
内侧前额叶皮层（mPFC）的前边缘（PL）区域与驱动和抑制动机行为有关，包括大鼠的可卡因寻求。这些看似相反的功能可能是由PL投射的不同输出靶点介导的，如伏隔核（NAc）核和前内侧被盖核（RMTg），它们在寻求奖励行为中起着截然不同的作用。我们试图描述投射到NAc核心的PL神经元和RMTg之间的解剖连通性差异。我们使用传统的逆行示踪剂来揭示大鼠中投射到NAc核心的PL神经元与RMTg的不同亚群，很少重叠。为了检验这两个PL亚群输入特异性的潜在差异，我们使用cre依赖性狂犬病毒（EnvA-RV-EGFP）作为单突触逆行示踪剂，通过在NAc核心或RMTg中注射逆行CAV2-Cre靶向特异性PL神经元。我们在NAc和rmtg投射的人群中观察到类似的皮层、丘脑和边缘传入事件目录，传入信息的主要来源来自同侧PL和边缘下皮层（IL）邻近的前额叶神经元。然而，当我们直接比较这两个亚群时，我们发现rmtg -突起的PL神经元从同侧PL和IL接收的输入比例更大，而nac -突起的PL神经元从大多数其他皮质区域、丘脑中背核和其他几个皮质下区域接收的输入比例更大。nac -突起的PL神经元也接受了更大比例的对侧皮质输入。我们的研究结果表明，PL亚群不仅在传入目标上不同，而且在传入结构的输入特异性上也不同。这些连通性的差异可能对PL亚群的功能差异至关重要。
The prelimbic (PL) region of the medial prefrontal cortex (mPFC) has been implicated in both driving and suppressing motivated behaviors, including cocaine-seeking in rats. These seemingly opposing functions may be mediated by different efferent targets of PL projections, such as the nucleus accumbens (NAc) core and rostromedial tegmental nucleus (RMTg), which have contrasting roles in reward-seeking behaviors. We sought to characterize the anatomical connectivity differences between PL neurons projecting to NAc core and RMTg. We used conventional retrograde tracers to reveal distinct subpopulations of PL neurons projecting to NAc core vs. RMTg in rats, with very little overlap. To examine potential differences in input specificity for these two PL subpopulations, we then used Cre-dependent rabies virus (EnvA-RV-EGFP) as a monosynaptic retrograde tracer and targeted specific PL neurons via injections of retrograde CAV2-Cre in either NAc core or RMTg. We observed a similar catalog of cortical, thalamic, and limbic afferents for both NAc- and RMTg-projecting populations, with the primary source of afferent information arising from neighboring prefrontal neurons in ipsilateral PL and infralimbic cortex (IL). However, when the two subpopulations were directly compared, we found that RMTg-projecting PL neurons received a greater proportion of input from ipsilateral PL and IL, whereas NAc-projecting PL neurons received a greater proportion of input from most other cortical areas, mediodorsal thalamic nucleus, and several other subcortical areas. NAc-projecting PL neurons also received a greater proportion of contralateral cortical input. Our findings reveal that PL subpopulations differ not only in their efferent target but also in the input specificity from afferent structures. These differences in connectivity are likely to be critical to functional differences of PL subpopulations.
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影响因子：
2.5
作者：
Dilgen, Jonathan;Tejeda, Hugo A.;O'Donnell, Patricio
通讯作者：
O'Donnell, Patricio
影响因子：
3.4
作者：
JAY, TM;THIERRY, AM;GLOWINSKI, J
通讯作者：
GLOWINSKI, J
影响因子：
2.5
作者：
Balcita-Pedicino, Judith Joyce;Omelchenko, Natalia;Bell, Roland;Sesack, Susan R.
通讯作者：
Sesack, Susan R.
影响因子：
7.7
作者：
Diehl MM;Iravedra-Garcia JM;Morán-Sierra J;Rojas-Bowe G;Gonzalez-Diaz FN;Valentín-Valentín VP;Quirk GJ
通讯作者：
Quirk GJ
影响因子：
5.3
作者：
Carr, DB;O'Donnell, P;Sesack, SR
通讯作者：
Sesack, SR