Microendoscopic calcium imaging of the primary visual cortex of behaving macaques.
Microendoscopic calcium imaging of the primary visual cortex of behaving macaques.
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DOI：
10.1038/s41598-021-96532-z
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发表时间：
2021-08-23
影响因子：
4.6
通讯作者：
Sakagami M
中科院分区：
文献类型：
--
作者：
Oguchi M;Jiasen J;Yoshioka TW;Tanaka YR;Inoue K;Takada M;Kikusui T;Nomoto K;Sakagami M
具有基因编码指标的体内钙成像最近已应用于猕猴大脑，以同时监测大量细胞的神经活动。显微内窥镜钙成像与植入式梯度折射率镜头相结合，通过紧凑便捷的设置捕获大脑深部区域的神经活动；然而，这仅限于啮齿动物和狨猴。在这里，我们开发了微型荧光显微镜来对行为猕猴初级视觉皮层的神经活动进行成像。我们发现来自六个大脑半球中的三个的数十个清晰的荧光信号。这些神经元的一个子集显示出清晰的视网膜位置和方向调节。此外，我们成功解码了刺激方向并跟踪了几天的细胞。这些结果表明，显微内窥镜钙成像通过监测大量神经元的荧光信号来研究猕猴大脑中的神经回路是可行且合理的。
In vivo calcium imaging with genetically encoded indicators has recently been applied to macaque brains to monitor neural activities from a large population of cells simultaneously. Microendoscopic calcium imaging combined with implantable gradient index lenses captures neural activities from deep brain areas with a compact and convenient setup; however, this has been limited to rodents and marmosets. Here, we developed miniature fluorescent microscopy to image neural activities from the primary visual cortex of behaving macaques. We found tens of clear fluorescent signals from three of the six brain hemispheres. A subset of these neurons showed clear retinotopy and orientation tuning. Moreover, we successfully decoded the stimulus orientation and tracked the cells across days. These results indicate that microendoscopic calcium imaging is feasible and reasonable for investigating neural circuits in the macaque brain by monitoring fluorescent signals from a large number of neurons.
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