Immune and Metabolic Biomarkers in a Rodent Model of Spinal Cord Contusion.
Immune and Metabolic Biomarkers in a Rodent Model of Spinal Cord Contusion.
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DOI：
10.1177/2192568220950337
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发表时间：
2022-01
影响因子：
2.4
通讯作者：
Grayson BE
中科院分区：
文献类型：
--
作者：
Dos Santos E Santos C;Welch BA;Edwards SR;Harris KK;Duncan BC;Himel AR;Grayson BE
基础科学动物研究。使用T10脊髓挫伤大鼠，我们试图确定分子和循环，代谢和免疫生物标志物在亚慢性和慢性恢复期，可能会告诉我们有关神经康复。在T10损伤大鼠中，在28和100天进行脊髓的基因表达和ELISA，并与假损伤大鼠进行比较。在恢复期后，将100天损伤的大鼠置于低脂肪或高脂肪饮食中。对标记物与运动评分、体重、身体组成、血胆固醇和甘油三酯之间进行线性回归分析。胸髓中补体标记物C1QC、树突状细胞标记物ITGAX和胆固醇生物合成基因FDFT 1、HMCGR、LDLR和SREBP 1的基因表达与BBB评分、体重、组成和其他代谢参数显著相关。然而，这些蛋白质的循环水平并没有因损伤而变化，也没有预测运动恢复的水平。鉴定可靠的循环生物标志物是持久的，并基于脊髓损伤的水平是复杂的免疫和代谢共病。继续工作是必要的，以确定稳定的疾病进展的标志物。
Basic science animal research study. Using T10 spinal contused rats, we sought to identify molecular and circulating, metabolic and immune biomarkers during the subchronic and chronic recovery periods that may inform us concerning neurorehabilitation. Gene expression of the cord and ELISA were performed in 28 and 100 days in T10 injured rats and compared to sham-injured rats. Hundred-day injured rats were placed on either a low-fat or high-fat diet following the recovery phase. Linear regression analysis was performed between markers and locomotor score, body weight, body composition, and blood cholesterol and triglycerides. Gene expression in the thoracic cord for complement marker, C1QC, dendritic cell marker, ITGAX, and cholesterol biosynthesis genes, FDFT1, HMCGR, LDLR, and SREBP1, were significantly associated with BBB score, body weight, composition, and other metabolic parameters. Circulating levels of these proteins, however, did not vary by injury or predict the level of locomotor recovery. Identification of reliable circulating biomarkers that are durable and based on level of spinal injury are complicated by immune and metabolic comorbidities. Continued work is necessary to identify stable markers of disease progression.
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影响因子：
1.2
作者：
Alves, Eduardo S;Santos, Ronaldo Vt;De Mello, Marco Tulio
通讯作者：
De Mello, Marco Tulio
影响因子：
4.2
作者：
Wang, Ruihua;Hong, Jun;Sheng, Huaxin
通讯作者：
Sheng, Huaxin
影响因子：
4.2
作者：
Scheff, SW;Rabchevsky, AG;Lumpp, JE
通讯作者：
Lumpp, JE
影响因子：
2.5
作者：
Harris, Kwamie K.;Himel, Alexandra R.;Grayson, Bernadette E.
通讯作者：
Grayson, Bernadette E.
影响因子：
4.1
作者：
Strimbu K;Tavel JA
通讯作者：
Tavel JA