Modulation of intestinal inflammation by yeasts and cell wall extracts: strain dependence and unexpected anti-inflammatory role of glucan fractions.
Modulation of intestinal inflammation by yeasts and cell wall extracts: strain dependence and unexpected anti-inflammatory role of glucan fractions.
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酵母和细胞壁提取物对肠道炎症的调节：葡萄糖级分的应变依赖性和意外的抗炎作用。
DOI：
10.1371/journal.pone.0040648
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Poulain D
中科院分区：
文献类型：
--
作者：
Jawhara S;Habib K;Maggiotto F;Pignede G;Vandekerckove P;Maes E;Dubuquoy L;Fontaine T;Guerardel Y;Poulain D
酵母菌及其聚糖成分对肠道炎症可能有有益或不利的影响。以前的研究表明，酿酒酵母变种的存在。布拉地（Sb）减少肠道炎症和白色念珠菌的定植。本研究的目的是确定饮食酵母，具有可比的效果，以抗C。白色念珠菌和Sb的抗炎特性，并评估酵母细胞壁组分调节肠道炎症的能力。小鼠接受C.白色念珠菌，然后给予1.5%葡聚糖硫酸钠（DSS）2周，随后是3天的恢复期。S.酿酒酵母菌株（Sb、Sc 1至Sc 4），以及由Sc2制备的甘露糖蛋白（MP）和β-葡聚糖粗级分和由C.在该治愈性模型中使用白色念珠菌，从C.白色念珠菌攻毒。评估小鼠与DSS给药相关的临床、组织学和炎症反应。菌株Sc 1 -1对C.通过死亡率、临床评分、定植水平、TNFα减少和IL-10转录增加评估时，将Sc 1 -1与其它S.酿酒酵母菌株，制备工艺对生物活性有很大影响。有趣的是，一些S。酿酒酵母菌株显著增加死亡率和临床评分。菌株Sc 4和MP组分对C.白念珠菌定植和炎症，而β-葡聚糖组分对两者都有保护作用。令人惊讶的是，从C.白色念珠菌具有相同的保护作用。因此，一些酵母似乎是肠道炎症的强调节剂。这些影响取决于菌株、种类、制备工艺和细胞壁分数。结果表明，C.白色念珠菌在DSS模型中显示出最有效的抗炎作用。
Yeasts and their glycan components can have a beneficial or adverse effect on intestinal inflammation. Previous research has shown that the presence of Saccharomyces cerevisiae var. boulardii (Sb) reduces intestinal inflammation and colonization by Candida albicans. The aim of this study was to identify dietary yeasts, which have comparable effects to the anti-C. albicans and anti-inflammatory properties of Sb and to assess the capabilities of yeast cell wall components to modulate intestinal inflammation. Mice received a single oral challenge of C. albicans and were then given 1.5% dextran-sulphate-sodium (DSS) for 2 weeks followed by a 3-day restitution period. S. cerevisiae strains (Sb, Sc1 to Sc4), as well as mannoprotein (MP) and β-glucan crude fractions prepared from Sc2 and highly purified β-glucans prepared from C. albicans were used in this curative model, starting 3 days after C. albicans challenge. Mice were assessed for the clinical, histological and inflammatory responses related to DSS administration. Strain Sc1-1 gave the same level of protection against C. albicans as Sb when assessed by mortality, clinical scores, colonization levels, reduction of TNFα and increase in IL-10 transcription. When Sc1-1 was compared with the other S. cerevisiae strains, the preparation process had a strong influence on biological activity. Interestingly, some S. cerevisiae strains dramatically increased mortality and clinical scores. Strain Sc4 and MP fraction favoured C. albicans colonization and inflammation, whereas β-glucan fraction was protective against both. Surprisingly, purified β-glucans from C. albicans had the same protective effect. Thus, some yeasts appear to be strong modulators of intestinal inflammation. These effects are dependent on the strain, species, preparation process and cell wall fraction. It was striking that β-glucan fractions or pure β-glucans from C. albicans displayed the most potent anti-inflammatory effect in the DSS model.
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影响因子：
29.4
作者：
Dalmasso, Guillaume;Cottrez, Francoise;Groux, Herve
通讯作者：
Groux, Herve
影响因子：
5.5
作者：
Cheng SC;van de Veerdonk FL;Lenardon M;Stoffels M;Plantinga T;Smeekens S;Rizzetto L;Mukaremera L;Preechasuth K;Cavalieri D;Kanneganti TD;van der Meer JW;Kullberg BJ;Joosten LA;Gow NA;Netea MG
通讯作者：
Netea MG
影响因子：
3.1
作者：
Fradin, C;Poulain, D;Jouault, T
通讯作者：
Jouault, T
影响因子：
4.2
作者：
Buddington, KK;Donahoo, JB;Buddington, RK
通讯作者：
Buddington, RK
影响因子：
3.7
作者：
Canonici, Alexandra;Siret, Carole;Andre, Frederic
通讯作者：
Andre, Frederic