Development of solid agents of the diphenyl ether herbicide degrading bacterium Bacillus sp. Za based on a mixed organic fertilizer carrier.
Development of solid agents of the diphenyl ether herbicide degrading bacterium Bacillus sp. Za based on a mixed organic fertilizer carrier.
复制标题
DOI：
10.3389/fmicb.2022.1075930
复制
发表时间：
2022
影响因子：
5.2
通讯作者：
Huang, Xing
中科院分区：
文献类型：
--
作者：
Zhao, Guoqiang;Tian, Yanning;Yu, Houyu;Li, Jintao;Mao, Dongmei;Faisal, Rayan Mazin;Huang, Xing
二苯醚除草剂的长期广泛使用造成了严重的土壤残留问题，威胁着农业生态环境。利用高效降解菌作为农药残留修复材料开发生物降解剂已得到广泛认可。本研究利用芽孢杆菌（Bacillus sp. Za）制备固体药剂对二苯醚除草剂污染土壤进行修复。有机肥配比为1:3（猪粪：牛粪），Za的接种量为10%，固体剂的施用量为7%，施用方式为混合施用，均为固体剂的最适宜条件。固体制剂保存120 d后，Za含量保持在108 CFU/g以上。在第7天，固体制剂对土壤中乳醚、联苯醚、氟糖醚和氟美沙芬的降解率分别达到87.40%、82.40%、78.20%和65.20%。固体药剂的施用减轻了乳醚残留对玉米幼苗的毒性作用。利用共聚焦激光扫描显微镜（CLSM）观察了Za-gfp在固体剂处理的玉米根表面的定殖情况，Za-gfp主要定殖在玉米根尖伸长区和成熟区，定殖时间超过21 d。对CK、J（固体剂）、Y（乳醚）和JY（固体剂+乳醚）组土壤群落结构变化的高通量测序分析表明，添加固体剂可以恢复玉米幼苗根际土壤细菌群落结构。固体药剂的开发有利于二苯醚除草剂残留污染土壤的修复，提高农药残留微生物降解的技术水平。
The long-term and widespread use of diphenyl ether herbicides has caused serious soil residue problems and threatens the agricultural ecological environment. The development of biodegrading agents using high-efficiency degrading strains as pesticide residue remediation materials has been widely recognized. In this study, the strain Bacillus sp. Za was used to prepare solid agents for the remediation of diphenyl ether herbicides-contaminated soil. The ratio of organic fertilizer was 1:3 (pig manure: cow dung), the inoculum amount of Za was 10%, the application amount of solid agents was 7%, and the application mode was mixed application, all of which were the most suitable conditions for solid agents. After the solid agents were stored for 120 days, the amount of Za remained above 108 CFU/g. The degradation rates of the solid agents for lactofen, bifenox, fluoroglycofen, and fomesafen in soil reached 87.40, 82.40, 78.20, and 65.20%, respectively, on the 7th day. The application of solid agents alleviated the toxic effect of lactofen residues on maize seedlings. A confocal laser scanning microscope (CLSM) was used to observe the colonization of Za-gfp on the surface of maize roots treated in the solid agents, and Za-gfp mainly colonized the elongation zone and the mature area of maize root tips, and the colonization time exceeded 21 days. High-throughput sequencing analysis of soil community structural changes in CK, J (solid agents), Y (lactofen), and JY (solid agents + lactofen) groups showed that the addition of solid agents could restore the bacterial community structure in the rhizosphere soil of maize seedlings. The development of solid agents can facilitate the remediation of soil contaminated with diphenyl ether herbicide residues and improve the technical level of the microbial degradation of pesticide residues.
登录
查看更多内容
影响因子：
2.8
作者：
More VS;Tallur PN;Niyonzima FN;More SS
通讯作者：
More SS
影响因子：
2.6
作者：
Chen, Liwei;Cai, Tianming;Wang, Qingling
通讯作者：
Wang, Qingling
新分离菌株 Bacillus sp FE-1 通过拟议的生化降解途径对氟磺胺草醚进行微生物降解和对氟磺胺草醚污染土壤的解毒
影响因子：
9.8
作者：
Cui, Ning;Wang, Saige;Yu, Yunlong
通讯作者：
Yu, Yunlong
影响因子：
6.1
作者：
Diao, Jinling;Lv, Chunguang;Zhou, Zhiqiang
通讯作者：
Zhou, Zhiqiang
通过氧化石墨烯固定细菌颗粒生物强化强化修复多环芳烃污染场地土壤
影响因子：
13.6
作者：
Ren, Wenjie;Liu, Haoran;Luo, Yongming
通讯作者：
Luo, Yongming