Magnetron sputtered diamond-like carbon microelectrodes for on-chip measurement of quantal catecholamine release from cells.
Magnetron sputtered diamond-like carbon microelectrodes for on-chip measurement of quantal catecholamine release from cells.
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DOI：
10.1007/s10544-008-9173-8
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发表时间：
2008-10
影响因子：
2.8
通讯作者：
Gangopadhyay S
中科院分区：
文献类型：
--
作者：
Gao Y;Chen X;Gupta S;Gillis KD;Gangopadhyay S
碳电极因其成本低、电位窗口宽、背景噪声低且稳定等优点而广泛应用于电化学领域。碳纤维电极（CFE）通常用于电化学测量通过单个细胞的胞吐作用释放的“量子”儿茶酚胺，但是难以将CFE集成到芯片实验室设备中。在这里，我们报告了氮掺杂的类金刚石碳（DLC：N）微电极的芯片上的发展，以监测从细胞中释放的儿茶酚胺的量子。DLC：N微电极的优点是它们可以低成本批量生产，并且比石墨膜更硬和更耐用。采用磁控溅射法制备了掺氮类金刚石膜（DLC：N）微电极。通过光刻和剥离技术将30 μm × 40 μm DLC：N微电极图案化到显微镜载玻片上。用原子力显微镜、拉曼光谱和循环伏安法对DLC：N微电极的性能进行了表征。在DLC：N微电极上，用电流法记录牛肾上腺嗜铬细胞的儿茶酚胺释放量。由于量子释放的儿茶酚胺的电流尖峰的振幅和面积类似的记录使用CFEs和背景电流和噪声水平的微芯片DLC：N电极的CFEs也相当。因此，DLC：N微电极适用于基于微芯片的量子胞吐的高通量测量，在基础研究、药物发现和基于细胞的生物传感器中具有应用。
Carbon electrodes are widely used in electrochemistry due to their low cost, wide potential window, and low and stable background noise. Carbon-fiber electrodes (CFE) are commonly used to electrochemically measure “quantal” catecholamine release via exocytosis from individual cells, but it is difficult to integrate CFEs into lab-on-a-chip devices. Here we report the development of nitrogen doped diamond-like carbon (DLC:N) microelectrodes on a chip to monitor quantal release of catecholamines from cells. Advantages of DLC:N microelectrodes are that they are batch producible at low cost, and are harder and more durable than graphite films. The DLC:N microelectrodes were prepared by a magnetron sputtering process with nitrogen doping. The 30 μm by 40 μm DLC:N microelectrodes were patterned onto microscope glass slides by photolithography and lift-off technology. The properties of the DLC:N microelectrodes were characterized by AFM, Raman spectroscopy and cyclic voltammetry. Quantal catecholamine release was recorded amperometrically from bovine adrenal chromaffin cells on the DLC:N microelectrodes. Amperometric spikes due to quantal release of catecholamines were similar in amplitude and area as those recorded using CFEs and the background current and noise levels of microchip DLC:N electrodes were also comparable to CFEs. Therefore, DLC:N microelectrodes are suitable for microchip-based high-throughput measurement of quantal exocytosis with applications in basic research, drug discovery and cell-based biosensors.
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影响因子：
3.7
作者：
Ferrari, AC;Robertson, J
通讯作者：
Robertson, J
影响因子：
3
作者：
Segura, F;Brioso, MA;Borges, R
通讯作者：
Borges, R
影响因子：
2.5
作者：
Gupta, S.;Patel, R. J.
通讯作者：
Patel, R. J.
影响因子：
6.1
作者：
Park, J;Jung, SH;Park, JO
通讯作者：
Park, JO
影响因子：
2.1
作者：
Sivertsen, JM;Wang, G;Judy, JH
通讯作者：
Judy, JH