System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidance.
System for delivering microwave ablation to subcutaneous tumors in small-animals under high-field MRI thermometry guidance.
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DOI：
10.1080/02656736.2022.2061727
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发表时间：
2022
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热疗后的生物效应是组织内实现的温度分布的函数，可以使用实时核磁共振测温(MRIT)来估计整个肿瘤体积。在这里，我们报告了一种先前开发的小动物微波热疗系统的扩展，该系统与MRIT相结合，用于对小鼠皮下移植肿瘤进行热消融。计算模型被用来评估2.45 GHz微波辐射器对小鼠皮下肿瘤靶点进行消融的适宜性。使用定制的微波辐射器将模体和体外组织加热到47-67°C的温度范围，以验证质子共振频率移动法与光纤测温的磁共振成像。将HAC15裸鼠移植瘤(n=6)体内消融，进行多平面MRIT监测。消融后一天，动物被安乐死，切除的肿瘤被处理以进行生存评估。在所有体外消融中，来自光纤传感器的温度与MRIT之间的平均绝对误差为0.6°C。在体内实验中，肿瘤体积在5.4-35.9mm3(平均14.2mm3)之间(持续时间：103mm3-150 S)被消融到肿瘤边界温度55°C。热剂量≥240CEM43覆盖了4例90.7-98.0%的肿瘤体积。其余病例的消融不完全，归因于运动影响的体温测量。基于热剂量的消融肿瘤覆盖率与切除肿瘤的生存评估相一致。我们已经开发了一种在MRIT指导下对小动物皮下肿瘤进行微波消融的系统，并在体内展示了它的性能。
Bio-effects following thermal treatments are a function of the achieved temperature profile in tissue, which can be estimated across tumor volumes with real-time MRI thermometry (MRIT). Here, we report on expansion of a previously developed small-animal microwave hyperthermia system integrated with MRIT for delivering thermal ablation to subcutaneously implanted tumors in mice. Computational models were employed to assess suitability of the 2.45 GHz microwave applicators for delivering ablation to subcutaneous tumor targets in mice. Phantoms and ex-vivo tissues were heated to temperatures in the range 47–67 °C with custom-made microwave applicators for validating MRIT with the proton resonance frequency shift method against fiberoptic thermometry. HAC15 tumors implanted in nude mice (n = 6) were ablated in vivo and monitored with MRIT in multiple planes. One day post ablation, animals were euthanized, and excised tumors were processed for viability assessment. Average absolute error between temperatures from fiberoptic sensors and MRIT was 0.6 °C across all ex-vivo ablations. During in-vivo experiments, tumors with volumes ranging between 5.4–35.9 mm3 (mean 14.2 mm3) were ablated (duration: 103–150 s) to achieve 55 °C at the tumor boundary. Thermal doses ≥240 CEM43 were achieved across 90.7–98.0% of tumor volumes for four cases. Ablations were incomplete for remaining cases, attributed to motion-affected thermometry. Thermal dose-based ablative tumor coverage agreed with viability assessment of excised tumors. We have developed a system for delivering microwave ablation to subcutaneous tumors in small animals under MRIT guidance and demonstrated its performance in-vivo.
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影响因子：
2.9
作者：
Lee, Kwang-Hun;Liapi, Eleni;Vossen, Josephina A.;Buijs, Manon;Ventura, Veronica Prieto;Georgiades, Christos;Hong, Kelvin;Kamel, Ihab;Torbenson, Michael S.;Geschwind, Jean-Francois H.
通讯作者：
Geschwind, Jean-Francois H.
影响因子：
3.1
作者：
Chen, Zubing;Shen, Shiqiang;Tao, Jianpin
通讯作者：
Tao, Jianpin
影响因子：
3.1
作者：
Amabile, Claudio;Ahmed, Muneeb;Goldberg, S. Nahum
通讯作者：
Goldberg, S. Nahum
影响因子：
3.8
作者：
Sebek, Jan;Albin, Nathan;Prakash, Punit
通讯作者：
Prakash, Punit
影响因子：
5.2
作者：
Priester MI;Curto S;Seynhaeve ALB;Perdomo AC;Amin M;Agnass P;Salimibani M;Faridi P;Prakash P;van Rhoon GC;Ten Hagen TLM
通讯作者：
Ten Hagen TLM