Preclinical Studies in Small Animals for Advanced Drug Delivery Using Hyperthermia and Intravital Microscopy.
Preclinical Studies in Small Animals for Advanced Drug Delivery Using Hyperthermia and Intravital Microscopy.
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DOI：
10.3390/cancers13205146
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发表时间：
2021-10-14
期刊：
影响因子：
5.2
通讯作者：
Ten Hagen TLM
中科院分区：
文献类型：
--
作者：
Priester MI;Curto S;Seynhaeve ALB;Perdomo AC;Amin M;Agnass P;Salimibani M;Faridi P;Prakash P;van Rhoon GC;Ten Hagen TLM
轻度热疗是一种诱导局部肿瘤温度升高至43.0 °C的技术。在本文中，我们介绍了三种设备，可用于在小动物中应用热疗结合活体显微镜在体内肿瘤内的事件，如药物输送的实时可视化。本文介绍了三种适合于临床前应用的热疗设备，通过同时高分辨率成像的肿瘤内事件。（预）临床研究已经证实，肿瘤微环境对局部轻度热疗的应用敏感。因此，加热是一种有前途的辅助放疗或化疗的疗效。更重要的是，轻度高温的应用是一个有用的刺激触发药物从热敏纳米载体释放。热敏纳米颗粒对高温的反应和随后的肿瘤内动力学在空间和时间上都相当复杂。为了更好地了解肿瘤内过程，纵向成像（最好在高空间和时间分辨率）是高度信息化的。我们的设备基于（i）用于背侧皮褶模型的外部电加热适配器，（ii）靶向射频应用，以及（iii）用于加热内部肿瘤的微波天线。这些模型，而一些技术上的复杂性，显着增加的理解的影响，轻度热疗抑制实施的研究热疗。
Mild hyperthermia is a technique that induces a local tumor temperature increase up to 43.0 °C. In this paper, we introduce three devices that could be used to apply hyperthermia in small animals in combination with intravital microscopy for the real-time visualization of in vivo intratumoral events such as drug delivery. This paper presents three devices suitable for the preclinical application of hyperthermia via the simultaneous high-resolution imaging of intratumoral events. (Pre)clinical studies have confirmed that the tumor micro-environment is sensitive to the application of local mild hyperthermia. Therefore, heating is a promising adjuvant to aid the efficacy of radiotherapy or chemotherapy. More so, the application of mild hyperthermia is a useful stimulus for triggered drug release from heat-sensitive nanocarriers. The response of thermosensitive nanoparticles to hyperthermia and ensuing intratumoral kinetics are considerably complex in both space and time. To obtain better insight into intratumoral processes, longitudinal imaging (preferable in high spatial and temporal resolution) is highly informative. Our devices are based on (i) an external electric heating adaptor for the dorsal skinfold model, (ii) targeted radiofrequency application, and (iii) a microwave antenna for heating of internal tumors. These models, while of some technical complexity, significantly add to the understanding of effects of mild hyperthermia warranting implementation in research on hyperthermia.
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影响因子：
16.1
作者：
Miller MA;Weissleder R
通讯作者：
Weissleder R
影响因子：
8
作者：
Kneidl B;Peller M;Winter G;Lindner LH;Hossann M
通讯作者：
Hossann M
影响因子：
15.9
作者：
Gul, Nuray;Babes, Liane;van Egmond, Marjolein
通讯作者：
van Egmond, Marjolein
DOI：
10.3109/02656730903124506
发表时间：
2009-11
期刊：
International journal of hyperthermia : the official journal of European Society for Hyperthermic Oncology, North American Hyperthermia Group
影响因子：
--
作者：
Arunachalam K;Maccarini PF;Schlorff JL;Birkelund Y;Jacobsen S;Stauffer PR
通讯作者：
Stauffer PR
DOI：
10.1007/bf01851841
发表时间：
1980-01-01
期刊：
RESEARCH IN EXPERIMENTAL MEDICINE
影响因子：
--
作者：
ENDRICH, B;ASAISHI, K;MESSMER, K
通讯作者：
MESSMER, K