Identification of an amphipathic helix important for the formation of ectopic septin spirals and axial budding in yeast axial landmark protein Bud3p.
Identification of an amphipathic helix important for the formation of ectopic septin spirals and axial budding in yeast axial landmark protein Bud3p.
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鉴定对酵母轴向标志蛋白 Bud3p 中异位 Septin 螺旋和轴向出芽重要的两亲螺旋
DOI：
10.1371/journal.pone.0016744
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发表时间：
2011-03-08
期刊：
影响因子：
3.7
通讯作者：
Gao XD
中科院分区：
文献类型：
--
作者：
Guo J;Gong T;Gao XD
极性轴的正确定位是细胞形态发生和细胞命运决定的核心。在芽殖酵母酿酒酵母中，Bud 3 p在轴向芽位选择（轴向芽殖）过程中起关键作用，在该过程中细胞在前一个细胞分裂位点旁边组装新芽。Bud 3 p被认为是空间地标的组成部分。然而，目前尚不清楚Bud 3 p如何与界标的其他组件（如隔膜）相互作用以控制轴向出芽。在这里，我们报告说，Bud 3 p的过度表达导致形成小隔蛋白环（直径约1 μm）和弧除了以前报道的螺旋状隔蛋白结构。Bud 3 p在体内与这些异常的septin结构共定位，并与两种septin Cdc 10 p和Cdc 11 p形成复合物，因此Bud 3 p与septins密切相关。Bud 3 p与septins的相互作用可能涉及Bud 3 p的多个区域，包括1-858、850-1220和1221-1636 a.a.因为它们都靶向芽颈，但当过表达时，对septin组织表现出不同的作用。此外，我们的研究表明，Bud 3 p的轴向出芽功能是由N端区域1-858介导的。该区域与参与异位隔蛋白螺旋和环的形成的中间部分850-1103共享对于芽颈靶向至关重要的两亲性螺旋（850-858）。有趣的是，位于1-858中的Dbl同源结构域对于轴向芽位点选择是有利的。我们的研究结果表明，Bud 3 p的多个区域确保Bud 3 p在轴向标志物的组装中有效地靶向芽颈，并且Bud 3 p的不同结构域参与轴向芽位点选择和其他细胞过程。
Correct positioning of polarity axis in response to internal or external cues is central to cellular morphogenesis and cell fate determination. In the budding yeast Saccharomyces cerevisiae, Bud3p plays a key role in the axial bud-site selection (axial budding) process in which cells assemble the new bud next to the preceding cell division site. Bud3p is thought to act as a component of a spatial landmark. However, it is not clear how Bud3p interacts with other components of the landmark, such as the septins, to control axial budding. Here, we report that overexpression of Bud3p causes the formation of small septin rings (∼1 µm in diameter) and arcs aside from previously reported spiral-like septin structures. Bud3p closely associates with the septins in vivo as Bud3p colocalizes with these aberrant septin structures and forms a complex with two septins, Cdc10p and Cdc11p. The interaction of Bud3p with the septins may involve multiple regions of Bud3p including 1–858, 850–1220, and 1221–1636 a.a. since they all target to the bud neck but exhibit different effects on septin organization when overexpressed. In addition, our study reveals that the axial budding function of Bud3p is mediated by the N-terminal region 1–858. This region shares an amphipathic helix (850–858) crucial for bud neck targeting with the middle portion 850–1103 involved in the formation of ectopic septin spirals and rings. Interestingly, the Dbl-homology domain located in 1–858 is dispensable for axial bud-site selection. Our findings suggest that multiple regions of Bud3p ensure efficient targeting of Bud3p to the bud neck in the assembly of the axial landmark and distinct domains of Bud3p are involved in axial bud-site selection and other cellular processes.
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影响因子：
2.9
作者：
Antonny, B;BeraudDufour, S;Chabre, M
通讯作者：
Chabre, M
影响因子：
56.9
作者：
Chen, T;Hiroko, T;Fujita, A
通讯作者：
Fujita, A
影响因子：
9.2
作者：
Lord, M;Inose, F;Chant, J
通讯作者：
Chant, J
DOI：
10.1083/jcb.151.7.1501
发表时间：
2000-12-25
期刊：
The Journal of cell biology
影响因子：
--
作者：
Lord M;Yang MC;Mischke M;Chant J
通讯作者：
Chant J
影响因子：
3.6
作者：
Justa-Schuch, Daniela;Heilig, Yvonne;Seiler, Stephan
通讯作者：
Seiler, Stephan