Self-assembly of DNA nanohydrogels with controllable size and stimuli-responsive property for targeted gene regulation therapy.
Self-assembly of DNA nanohydrogels with controllable size and stimuli-responsive property for targeted gene regulation therapy.
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DOI：
10.1021/ja512293f
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发表时间：
2015-02-04
影响因子：
15
通讯作者：
Tan, Weihong
中科院分区：
文献类型：
--
作者：
Li, Juan;Zheng, Cheng;Cansiz, Sena;Wu, Cuichen;Xu, Jiehua;Cui, Cheng;Liu, Yuan;Hou, Weijia;Wang, Yanyue;Zhang, Liqin;Teng, I-ting;Yang, Huang-Hao;Tan, Weihong
在这里，我们报道了尺寸可控和刺激响应的DNA纳米水凝胶作为有效的靶向基因传递载体的合成和表征。DNA纳米水凝胶是由三种不同的结构单元通过自组装过程形成的，分别称为带有三个粘性末端的Y形单体A(YMA)、带有一个粘性末端的Y形单体B(YMB)和带有两个粘性末端的DNA连接物(LK)。单体和LK粘性末端的杂交导致纳米水凝胶的形成。通过改变YMA和YMB的比例，DNA纳米水凝胶的尺寸是可控制的。通过将不同的功能元件，如适配子、二硫键和治疗性基因整合到不同的构建单元中，合成的基于适体的纳米水凝胶(Y-Gel-APT)可以用于靶向和刺激响应性的基因治疗。Y-Gel-APT对靶细胞A549的增殖和迁移有明显的抑制作用，但对对照细胞无明显抑制作用。这种Y-Gel-APT利用了简单的模块化设计和组装、高效的细胞摄取和卓越的生物兼容性，有望成为靶向基因或药物输送和癌症治疗的候选药物。
Here, we report the synthesis and characterization of size-controllable and stimuli-responsive DNA nanohydrogels as effective targeted gene delivery vectors. DNA nanohydrogels were created through a self-assembly process using three kinds of building units, respectively termed Y-shaped monomer A with three sticky ends (YMA), Y-shaped monomer B with one sticky end (YMB), and DNA linker (LK) with two sticky ends. Hybridization at the sticky ends of monomers and LK leads to nanohydrogel formation. DNA nanohydrogels are size-controllable by varying the ratio of YMA to YMB. By incorporating different functional elements, such as aptamers, disulfide linkages, and therapeutic genes into different building units, the synthesized aptamer-based nanohydrogels (Y-gel-Apt) can be used for targeted and stimuli-responsive gene therapy. Y-gel-Apt strongly inhibited cell proliferation and migration in target A549 cells, but not in control cells. By taking advantage of facile modular design and assembly, efficient cellular uptake, and superior biocompatibility, this Y-gel-Apt holds great promise as a candidate for targeted gene or drug delivery and cancer therapy.
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影响因子：
15
作者：
Dunn, Stuart S.;Tian, Shaomin;Blake, Steven;Wang, Jin;Galloway, Ashley L.;Murphy, Andrew;Pohlhaus, Patrick D.;Rolland, Jason P.;Napier, Mary E.;DeSimone, Joseph M.
通讯作者：
DeSimone, Joseph M.
影响因子：
15
作者：
Yang, Huanghao;Liu, Haipeng;Tan, Weihong
通讯作者：
Tan, Weihong
影响因子：
15
作者：
Yan, Ling;Zhu, Zhi;Yang, Chaoyong James
通讯作者：
Yang, Chaoyong James
影响因子：
4.2
作者：
Wu, GY;Fang, YZ;Turner, ND
通讯作者：
Turner, ND
pH 触发、快速响应的 DNA 水凝胶
影响因子：
16.6
作者：
Cheng, Enjun;Xing, Yongzheng;Liu, Dongsheng
通讯作者：
Liu, Dongsheng