L-selectin shedding does not regulate constitutive T cell trafficking but controls the migration pathways of antigen-activated T lymphocytes.
L-selectin shedding does not regulate constitutive T cell trafficking but controls the migration pathways of antigen-activated T lymphocytes.
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DOI：
10.1084/jem.20030485
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发表时间：
2003-11-03
影响因子：
15.3
通讯作者：
Ager, A
中科院分区：
文献类型：
--
作者：
Galkina, E;Tanousis, K;Preece, G;Tolaini, M;Kioussis, D;Florey, O;Haskard, DO;Tedder, TF;Ager, A
L-Selectin mediates rolling of lymphocytes in high endothelial venules (HEVs) of peripheral lymph nodes (PLNs). Cross-linking of L-selectin causes proteolytic shedding of its ectodomain, the physiological significance of which is unknown. To determine whether L-selectin shedding regulates lymphocyte migration, a mutant form that resists shedding (LΔP-selectin) was engineered. Transgenic mice expressing either LΔP or wild-type (WT) L-selectin on T cells were crossed with L-selectin knockout (KO) mice. The cellularity and subset composition of secondary lymphoid organs did not differ between LΔP and WT mice, however, they were different from C57BL/6. Plasma levels of soluble L-selectin in LΔP mice were reduced to <5% of WT and C57BL/6 mice. The rolling properties of T lymphocytes from LΔP and WT mice on immobilized L-selectin ligands were similar. Furthermore, similar numbers of LΔP and WT T lymphocytes were recruited from the bloodstream into PLNs in mice, although LΔP T cells transmigrated HEVs more slowly. WT, but not LΔP-selectin, underwent rapid, metalloproteinase-dependent shedding after TCR engagement, and LΔP T cells retained the capacity to enter PLNs from the bloodstream. These results suggest that the ability to shed L-selectin is not required for T cell recirculation and homing to PLNs. However, L-selectin shedding from antigen-activated T cells prevents reentry into PLNs.
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DOI：
10.1083/jcb.138.5.1169
发表时间：
1997-09-08
期刊：
The Journal of cell biology
影响因子：
--
作者：
Alon R;Chen S;Puri KD;Finger EB;Springer TA
通讯作者：
Springer TA
影响因子：
56.9
作者：
Butcher, EC;Picker, LJ
通讯作者：
Picker, LJ
影响因子：
3.5
作者：
Amour, A;Slocombe, PM;Murphy, G
通讯作者：
Murphy, G
DOI：
10.1084/jem.193.8.987
发表时间：
2001-04-16
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Iezzi G;Scheidegger D;Lanzavecchia A
通讯作者：
Lanzavecchia A
影响因子：
64.5
作者：
Förster, R;Schubel, A;Lipp, M
通讯作者：
Lipp, M