The Importance of Evaluating the Lot-to-Lot Batch Consistency of Commercial Multi-Walled Carbon Nanotube Products.
The Importance of Evaluating the Lot-to-Lot Batch Consistency of Commercial Multi-Walled Carbon Nanotube Products.
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DOI：
10.3390/nano10101930
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发表时间：
2020-09-27
期刊：
影响因子：
--
通讯作者：
Pantano P
中科院分区：
文献类型：
--
作者：
Huynh MT;Veyan JF;Pham H;Rahman R;Yousuf S;Brown A;Lin J;Balkus KJ Jr;Diwakara SD;Smaldone RA;LeGrand B;Mikoryak C;Draper R;Pantano P
多壁碳纳米管(MWNTs)的生物响应与其物理化学性质有关，在评估其生物活性的同时，还应对其进行彻底的表征，包括潜在的毒性。除了表征来自不同来源或制造商的多壁碳纳米管的物理化学性质外，还必须表征来自同一供应商的同一多壁碳纳米管产品的不同生产批次(即批次到批次的一致性)。在此，我们对两批商用原始多壁碳纳米管(PMWNTs)和羧化多壁碳纳米管(CMWNTs)进行了全面的物理化学表征，用于研究哺乳动物巨噬细胞对多壁碳纳米管的反应。两批cMWNTs的物理化学性质有许多相似之处，但都没有显著抑制原始264.7巨噬细胞24小时的增殖，直到测试的最高浓度(200cMWNTs/mL)。相反，两批pMWNTs的几个物理化学性质不同；值得注意的是，新一批pMWNTs的氧化稳定性较差，缺陷密度较高，表面氧物种数量较少。此外，新批次的pMWNTs对RAW 264.7细胞的72 h半数抑制浓度(IC-50)为~90µg pMWNTs/mL。这些结果表明，细微的物理化学差异可能导致显著不同的细胞反应，在评估MWNTs的毒性时必须考虑生产批次的一致性。
The biological response of multi-walled carbon nanotubes (MWNTs) is related to their physicochemical properties and a thorough MWNT characterization should accompany an assessment of their biological activity, including their potential toxicity. Beyond characterizing the physicochemical properties of MWNTs from different sources or manufacturers, it is also important to characterize different production lots of the same MWNT product from the same vendor (i.e., lot-to-lot batch consistency). Herein, we present a comprehensive physicochemical characterization of two lots of commercial pristine MWNTs (pMWNTs) and carboxylated MWNTs (cMWNTs) used to study the response of mammalian macrophages to MWNTs. There were many similarities between the physicochemical properties of the two lots of cMWNTs and neither significantly diminished the 24-h proliferation of RAW 264.7 macrophages up to the highest concentration tested (200 μg cMWNTs/mL). Conversely, several physicochemical properties of the two lots of pMWNTs were different; notably, the newer lot of pMWNTs displayed less oxidative stability, a higher defect density, and a smaller amount of surface oxygen species relative to the original lot. Furthermore, a 72-h half maximal inhibitory concentration (IC-50) of ~90 µg pMWNTs/mL was determined for RAW 264.7 cells with the new lot of pMWNTs. These results demonstrate that subtle physicochemical differences can lead to significantly dissimilar cellular responses, and that production-lot consistency must be considered when assessing the toxicity of MWNTs.
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影响因子：
14
作者：
Chen, Shu;Hu, Sheng;Smith, Elizabeth F.;Ruenraroengsak, Pakatip;Thorley, Andrew J.;Menzel, Robert;Goode, Angela E.;Ryan, Mary P.;Tetley, Teresa D.;Porter, Alexandra E.;Shaffer, Milo S. P.
通讯作者：
Shaffer, Milo S. P.
镍纳米粒子和碳纳米管负载的碳包覆镍纳米粒子的合成
影响因子：
3.3
作者：
Cheng, JP;Zhang, XB;Ye, Y
通讯作者：
Ye, Y
DOI：
10.1080/15459624.2016.1191639
发表时间：
2016-12
影响因子：
2
作者：
Braun EI;Huang A;Tusa CA;Yukica MA;Pantano P
通讯作者：
Pantano P
影响因子：
10.9
作者：
Andrews, R;Jacques, D;Dickey, EC
通讯作者：
Dickey, EC
影响因子：
4.3
作者：
Boverhof, Darrell R.;David, Raymond M.
通讯作者：
David, Raymond M.