Degradation and Detoxification of Aflatoxin B1 by Tea-Derived Aspergillus niger RAF106.
Degradation and Detoxification of Aflatoxin B1 by Tea-Derived Aspergillus niger RAF106.
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茶源黑曲霉RAF106对黄曲霉毒素B1的降解和解毒
DOI：
10.3390/toxins12120777
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发表时间：
2020-12-06
期刊：
影响因子：
4.2
通讯作者：
Wang J
中科院分区：
文献类型：
--
作者：
Fang Q;Du M;Chen J;Liu T;Zheng Y;Liao Z;Zhong Q;Wang L;Fang X;Wang J
黄曲霉毒素B1（AFB 1）对人畜具有严重的毒性，微生物降解是一种有效的去除方法。在黄曲霉毒素B1浓度为0.1 ~ 4 μg/mL的沙氏葡萄糖肉汤（SDB）中，尼日尔RAF 106能有效降解黄曲霉毒素B1。以酵母膏为氮源的处理促进了降解，而以NaNO 3和NaNO 2为氮源，乳糖和蔗糖为碳源的处理抑制了降解。此外，A.尼日尔RAF 106在初始pH值为4 ~ 10、培养温度为25 ~ 45 °C时仍能降解黄曲霉毒素B1。此外，具有优异耐热性的细胞内酶或蛋白质被证实能够将AFB 1降解成具有低致突变性或无致突变性的代谢产物。此外，基因组序列分析表明，该真菌被认为是安全的，因为没有毒力基因和合成真菌毒素的基因簇。这些结果表明A.尼日尔RAF 106及其胞内酶或蛋白在食品和饲料加工和工业中具有很好的应用前景，可用于AFB 1的脱毒。
Microbial degradation is an effective and attractive method for eliminating aflatoxin B1 (AFB1), which is severely toxic to humans and animals. In this study, Aspergillus niger RAF106 could effectively degrade AFB1 when cultivated in Sabouraud dextrose broth (SDB) with contents of AFB1 ranging from 0.1 to 4 μg/mL. Treatment with yeast extract as a nitrogen source stimulated the degradation, but treatment with NaNO3 and NaNO2 as nitrogen sources and lactose and sucrose as carbon sources suppressed the degradation. Moreover, A. niger RAF106 still degraded AFB1 at initial pH values that ranged from 4 to 10 and at cultivation temperatures that ranged from 25 to 45 °C. In addition, intracellular enzymes or proteins with excellent thermotolerance were verified as being able to degrade AFB1 into metabolites with low or no mutagenicity. Furthermore, genomic sequence analysis indicated that the fungus was considered to be safe owing to the absence of virulence genes and the gene clusters for the synthesis of mycotoxins. These results indicate that A. niger RAF106 and its intracellular enzymes or proteins have a promising potential to be applied commercially in the processing and industry of food and feed to detoxify AFB1.
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影响因子：
3.7
作者：
Colin VL;Baigorí MD;Pera LM
通讯作者：
Pera LM
尼日尔菌株AN76的基因组序列草案。
DOI：
10.1128/genomea.01700-15
发表时间：
2016-02-18
期刊：
Genome announcements
影响因子：
--
作者：
Gong W;Cheng Z;Zhang H;Liu L;Gao P;Wang L
通讯作者：
Wang L
影响因子：
6
作者：
Farzaneh, Mohsen;Shi, Zhi-Qi;Javan-Nikkhah, Mohammad
通讯作者：
Javan-Nikkhah, Mohammad
影响因子：
3
作者：
Lahoum, Abdelhadi;Verheecke-Vaessen, Carol;Mathieu, Florence
通讯作者：
Mathieu, Florence
DOI：
10.1073/pnas.70.8.2281
发表时间：
1973-01-01
影响因子：
11.1
作者：
AMES, BN;DURSTON, WE;LEE, FD
通讯作者：
LEE, FD