Cell contact-dependent outer membrane exchange in myxobacteria: genetic determinants and mechanism.
Cell contact-dependent outer membrane exchange in myxobacteria: genetic determinants and mechanism.
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DOI：
10.1371/journal.pgen.1002626
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发表时间：
2012
期刊：
影响因子：
4.5
通讯作者：
Wall D
中科院分区：
文献类型：
--
作者：
Pathak DT;Wei X;Bucuvalas A;Haft DH;Gerloff DL;Wall D
生物膜是密集的微生物群落，尽管分布广泛，并且在医学上很重要，但生物膜细胞彼此之间的相互作用很差。在这里，这些蛋白质需要的两个宿主蛋白质被预测在细胞包膜中遥远的PA14讲座样域，富含半胱氨酸的串联重复区域以及一个名为Myxo-cerm的推定的C端蛋白排序标签，而Trab编码供体和受体中的TRAAB是必需的。双向转移。运动性和结构化生物膜。哪些脂蛋白/脂质是通过横向扩散转移的。
所有细胞与其他细胞（包括其他细胞）相互作用，引起细胞 - 细胞之间的相互作用。由于这些生物相对简单，并且通常被描述为单细胞实体。社区或生物膜作为一种主要的生活方式而被广泛接受，因为生物膜细胞被紧密地接触，但有机会提供相邻细胞的空间和物理线索，尽管已经学会了很多。关于通过法定传感的扩散信号，对于细菌中的细胞接触依赖性信号，我们描述了一种新机制。结构化的生物膜内的细菌细胞形成允许交换细胞材料的接触，这会引起表型变化，包括在细胞运动和发育中。
Biofilms are dense microbial communities. Although widely distributed and medically important, how biofilm cells interact with one another is poorly understood. Recently, we described a novel process whereby myxobacterial biofilm cells exchange their outer membrane (OM) lipoproteins. For the first time we report here the identification of two host proteins, TraAB, required for transfer. These proteins are predicted to localize in the cell envelope; and TraA encodes a distant PA14 lectin-like domain, a cysteine-rich tandem repeat region, and a putative C-terminal protein sorting tag named MYXO-CTERM, while TraB encodes an OmpA-like domain. Importantly, TraAB are required in donors and recipients, suggesting bidirectional transfer. By use of a lipophilic fluorescent dye, we also discovered that OM lipids are exchanged. Similar to lipoproteins, dye transfer requires TraAB function, gliding motility and a structured biofilm. Importantly, OM exchange was found to regulate swarming and development behaviors, suggesting a new role in cell–cell communication. A working model proposes TraA is a cell surface receptor that mediates cell–cell adhesion for OM fusion, in which lipoproteins/lipids are transferred by lateral diffusion. We further hypothesize that cell contact–dependent exchange helps myxobacteria to coordinate their social behaviors. All cells interact with their environment, including other cells, to elicit cellular responses. Cell–cell interactions between eukaryotic cells are widely appreciated as large multicellular organisms coordinate cell behaviors for tissue and organ functions. In bacteria cell–cell interactions are not widely appreciated, as these organisms are relatively simple and are often depicted as single-cell entities. However, over the past decade, the concept of bacteria living in microbial communities or biofilms has received broad acceptance as a major lifestyle. As biofilm cells are packed in tight physical contact, there is an opportunity for cell–cell signaling to provide spatial and physiological clues of neighboring cells to elicit cellular responses. Although much has been learned about diffusible signals through quorum sensing, little is known about cell contact–dependent signaling in bacteria. In this report we describe a new mechanism where bacterial cells within structured biofilms form contacts that allow cellular material to be exchanged. This exchange elicits phenotypic changes, including in cell movements and development. We hypothesize that OM exchange involves kin recognition that bestows social benefits to myxobacterial populations.
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影响因子：
2.9
作者：
Krivov, Georgii G.;Shapovalov, Maxim V.;Dunbrack, Roland L., Jr.
通讯作者：
Dunbrack, Roland L., Jr.
影响因子：
10.5
作者：
KIM, SK;KAISER, D
通讯作者：
KAISER, D
影响因子：
14.9
作者：
Biegert A;Mayer C;Remmert M;Söding J;Lupas AN
通讯作者：
Lupas AN
DOI：
10.1073/pnas.96.14.8052
发表时间：
1999-07-06
影响因子：
11.1
作者：
Fontes, M;Kaiser, D
通讯作者：
Kaiser, D
影响因子：
3.3
作者：
Gardner, Andy;West, Stuart A.
通讯作者：
West, Stuart A.