High proportions of pfhrp2 gene deletion and performance of HRP2-based rapid diagnostic test in Plasmodium falciparum field isolates of Odisha.
High proportions of pfhrp2 gene deletion and performance of HRP2-based rapid diagnostic test in Plasmodium falciparum field isolates of Odisha.
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DOI：
10.1186/s12936-018-2502-3
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发表时间：
2018-10-29
期刊：
影响因子：
3
通讯作者：
Ranjit M
中科院分区：
文献类型：
--
作者：
Pati P;Dhangadamajhi G;Bal M;Ranjit M
随着快速诊断试验（RDT）阴性恶性疟疾病例的记录，尽管在奥里萨邦的一个小口袋中自然分离的频率很低，但在整个邦和一年中的不同季节调查基于酶标2的RDT的状况是绝对必要的。在印度奥里萨邦25/30个区，用显微镜和RDT对疑似个体进行了疟疾感染筛查。PCR证实了结果的差异。采用ELISA法检测单恶性疟原虫假阴性RDT标本的HRP2抗原，并对pfhrp2、pfhrp3及其侧翼基因进行分型。通过msp1和msp2基因分型确定感染的多重性，镜检检测寄生虫血症水平。在1058例疑似疟疾患者中，384例镜检确诊为单恶性疟原虫感染，在该州不同地区的58个样本中观察到不同比例的RDT失败。检测失败的原因是无法检测到HRP-2的水平。虽然大部分样本是在雨季筛选的（45/345），但与雨季相比，夏季获得的RDT阴性样本比例（9/17）显著高于夏季（P = 0.0002; OR = 7.5）。对RDT阴性样本pfhrp2和pfhrp3的PCR基因分型结果显示，38/58（65.5）份样本pfhrp2阴性，24/58（41.4）份样本pfhrp3阴性，其中17/58（29.3）份样本pfhrp3双阴性。大多数RDT阴性样本（39/58）为单基因型感染，pfhrp2缺失比例较高（7/9）。RDT阳性和RDT阴性患者的寄生虫血症水平无差异。伴有pfhrp2缺失（包括双缺失（pfhrp2和pfhrp3））的寄生虫的高流行率是一个严重的问题，因为这些患者无法得到正确的诊断和治疗。因此，基于hrp2的RDT诊断奥里萨邦恶性疟原虫感染是不可靠的，必须与其他适当的诊断工具一起进行或被其他适当的诊断工具取代，以进行疾病的临床管理。本文的在线版本（10.1186/s12936-018-2502-3）包含补充材料，授权用户可使用。
With the documentation of cases of falciparum malaria negative by rapid diagnostic tests (RDT), though at low frequency from natural isolates in a small pocket of Odisha, it became absolutely necessary to investigate the status of HRP-2 based RDT throughout the state and in different seasons of the year. Suspected individuals were screened for malaria infection by microscopy and RDT in 25/30 districts of Odisha, India. Discrepancies in results were confirmed by PCR. False negative RDT samples for Plasmodium falciparum mono-infection were evaluated for detection of HRP2 antigen in ELISA and genotyped for pfhrp2, pfhrp3 and their flanking genes. Multiplicity of infection was ascertained based on msp1 and msp2 genotyping and parasitaemia level was determined by microscopy. Of the total 1058 patients suspected for malaria, 384 were microscopically confirmed for P. falciparum mono-infection and RDT failure was observed in 58 samples at varying proportion in different regions of the state. The failure in detection was due to undetectable level of HRP-2. Although most of these samples were screened during rainy season (45/345), significantly high proportion (9/17) of RDT negative samples were obtained during the summer compared to rainy season (P = 0.0002; OR = 7.5). PCR genotyping of pfhrp2 and pfhrp3 in RDT negative samples showed 38/58 (65.5) samples to be pfhrp2 negative and 24/58 (41.4) to be pfhrp3 negative including dual negative in 17/58 (29.3). Most of the RDT negative samples (39/58) were with single genotype infection and high proportions of pfhrp2 deletion (7/9) was observed in summer. No difference in parasitaemia level was observed between RDT positive and RDT negative patients. High prevalence of parasites with pfhrp2 deletion including dual deletions (pfhrp2 and pfhrp3) is a serious cause of concern, as these patients could not be given a correct diagnosis and treatment. Therefore, HRP2-based RDT for diagnosing P. falciparum infection in Odisha is non-reliable and must be performed in addition to or replaced by other appropriate diagnostic tools for clinical management of the disease. The online version of this article (10.1186/s12936-018-2502-3) contains supplementary material, which is available to authorized users.
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DOI：
10.1083/jcb.103.4.1269
发表时间：
1986-10
期刊：
The Journal of cell biology
影响因子：
--
作者：
Howard RJ;Uni S;Aikawa M;Aley SB;Leech JH;Lew AM;Wellems TE;Rener J;Taylor DW
通讯作者：
Taylor DW
影响因子：
3
作者：
Kyabayinze DJ;Tibenderana JK;Odong GW;Rwakimari JB;Counihan H
通讯作者：
Counihan H
影响因子：
6.4
作者：
Hopkins, Heidi;Bebell, Lisa;Dorsey, Grant
通讯作者：
Dorsey, Grant
影响因子：
3
作者：
Diarra, Amidou;Nebie, Issa;Sirima, Sodiomon B.
通讯作者：
Sirima, Sodiomon B.
影响因子：
3.7
作者：
Gamboa D;Ho MF;Bendezu J;Torres K;Chiodini PL;Barnwell JW;Incardona S;Perkins M;Bell D;McCarthy J;Cheng Q
通讯作者：
Cheng Q