Bovicin HJ50-like lantibiotics, a novel subgroup of lantibiotics featured by an indispensable disulfide bridge.
Bovicin HJ50-like lantibiotics, a novel subgroup of lantibiotics featured by an indispensable disulfide bridge.
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Bovicin HJ50 类羊毛硫抗生素，一种以不可或缺的二硫桥为特征的新型羊毛硫抗生素亚类
DOI：
10.1371/journal.pone.0097121
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发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Zhong J
中科院分区：
文献类型：
--
作者：
Wang J;Ma H;Ge X;Zhang J;Teng K;Sun Z;Zhong J
抗生素是一类核糖体合成和翻译后修饰的多肽，具有很强的抗菌活性。随着微生物基因组信息的迅速发布，新型抗生素的发现大大加快了步伐。博维菌素HJ50是由牛链球菌HJ50产生的一种独特的II类抗生素，它含有一个罕见的二硫键。以其前体Bova为驱动序列，在多种物种中发现了16个Bova样多肽。从中筛选出产气荚膜梭菌的3个有代表性的新基因座。经聚合酶链式反应鉴定为JGS1721、蜡状芽孢杆菌1.348和苏云金芽孢杆菌1.013。得到了相应的成熟抗生素，命名为Perecin、Cerecin和Thuricin，结构鉴定为bovicin HJ50类抗生素，尤其是含有保守的二硫键。二硫键被证实对Bovicin HJ50类抗生素的功能是必不可少的，因为它的破坏消除了它们的抗菌活性。进一步的分析表明，二硫键在保持bovicin HJ50的疏水性方面起着关键作用，这可能有助于其发挥抗菌功能。本研究揭示了来自不同生态位细菌的一个新的含二硫键的抗生素亚群，并进一步证明了二硫键在这些新的牛乳素HJ50类抗生素中所起的不可或缺的作用。
Lantibiotics are ribosomally-synthesized and posttranslationally modified peptides with potent antimicrobial activities. Discovery of novel lantibiotics has been greatly accelerated with the soaring release of genomic information of microorganisms. As a unique class II lantibiotic, bovicin HJ50 is produced by Streptococcus bovis HJ50 and contains one rare disulfide bridge. By using its precursor BovA as a drive sequence, 16 BovA-like peptides were revealed in a wide variety of species. From them, three representative novel lan loci from Clostridium perfringens D str. JGS1721, Bacillus cereus As 1.348 and B. thuringiensis As 1.013 were identified by PCR screening. The corresponding mature lantibiotics designated perecin, cerecin and thuricin were obtained and structurally elucidated to be bovicin HJ50-like lantibiotics especially by containing a conserved disulfide bridge. The disulfide bridge was substantiated to be essential for the function of bovicin HJ50-like lantibiotics as its disruption eliminated their antimicrobial activities. Further analysis indicated that the disulfide bridge played a crucial role in maintaining the hydrophobicity of bovicin HJ50, which might facilitate it to exert antimicrobial function. This study unveiled a novel subgroup of disulfide-containing lantibiotics from bacteria of different niches and further demonstrated the indispensable role of disulfide bridge in these novel bovicin HJ50-like lantibiotics.
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影响因子：
15
作者：
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