Herbicide ingredients change Salmonella enterica sv. Typhimurium and Escherichia coli antibiotic responses.
Herbicide ingredients change Salmonella enterica sv. Typhimurium and Escherichia coli antibiotic responses.
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DOI：
10.1099/mic.0.000573
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发表时间：
2017-12
期刊：
影响因子：
--
通讯作者：
Heinemann JA
中科院分区：
文献类型：
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作者：
Kurenbach B;Gibson PS;Hill AM;Bitzer AS;Silby MW;Godsoe W;Heinemann JA
除草剂经常被释放到农村和城市环境中。商业除草剂制剂诱导细菌对抗生素反应方式的适应性变化。肠沙门氏菌将鼠伤寒杆菌和大肠杆菌暴露于常用的复方制剂中，并将S.肠道将鼠伤寒沙门氏菌暴露于活性成分麦草畏、2，4-D和草甘膦，以确定商业制剂中的哪些成分引起这种作用。复方制剂Tween 80和羧甲基纤维素诱导了反应的变化，但反应的模式与活性成分不同，且效应量较小。市售润湿剂不影响抗生素反应。活性成分诱导的抗生素反应变化与完整制剂引起的变化相似。在推荐的应用浓度或低于推荐的应用浓度时发生这种情况。靶向缺失外排泵基因在很大程度上中和了抗生素存活率增加病例中的适应性反应，表明诱导耐药的生化过程与制剂和特定成分相同。我们发现草甘膦、麦草畏和2，4-D以及商业除草剂中的共制剂诱导潜在病原菌E. coli和革兰氏阳性菌S.肠道感染多种抗生素。这是使用平板效率（EOP）来测量的，即当暴露于除草剂和抗生素或仅暴露于抗生素时细菌的相对存活率与在允许培养基上的存活率相比。这项工作将有助于告知使用非药用化学试剂，诱导抗生素反应的变化。
Herbicides are frequently released into both rural and urban environments. Commercial herbicide formulations induce adaptive changes in the way bacteria respond to antibiotics. Salmonella enterica sv. Typhimurium and Escherichia coli were exposed to common co-formulants of formulations, and S. enterica sv. Typhimurium was exposed to active ingredients dicamba, 2,4-D and glyphosate to determine what ingredients of the commercial formulations caused this effect. Co-formulants Tween80 and carboxymethyl cellulose induced changes in response, but the pattern of the responses differed from the active ingredients, and effect sizes were smaller. A commercial wetting agent did not affect antibiotic responses. Active ingredients induced changes in antibiotic responses similar to those caused by complete formulations. This occurred at or below recommended application concentrations. Targeted deletion of efflux pump genes largely neutralized the adaptive response in the cases of increased survival in antibiotics, indicating that the biochemistry of induced resistance was the same for formulations and specific ingredients. We found that glyphosate, dicamba, and 2,4-D, as well as co-formulants in commercial herbicides, induced a change in susceptibility of the potentially pathogenic bacteria E. coli and S. enterica to multiple antibiotics. This was measured using the efficiency of plating (EOP), the relative survival of the bacteria when exposed to herbicide and antibiotic, or just antibiotic, compared to survival on permissive media. This work will help to inform the use of non-medicinal chemical agents that induce changes in antibiotic responses.
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影响因子：
6.4
作者：
Kurenbach B;Marjoshi D;Amábile-Cuevas CF;Ferguson GC;Godsoe W;Gibson P;Heinemann JA
通讯作者：
Heinemann JA
影响因子：
64.8
作者：
Chassaing B;Koren O;Goodrich JK;Poole AC;Srinivasan S;Ley RE;Gewirtz AT
通讯作者：
Gewirtz AT
DOI：
10.1016/j.ijantimicag.2013.07.008
发表时间：
2013-11-01
影响因子：
10.8
作者：
Ferran, Aude A.;Bibbal, Delphine;Bousquet-Melou, Alain
通讯作者：
Bousquet-Melou, Alain
影响因子：
3.2
作者：
COHEN, SP;LEVY, SB;ROSNER, JL
通讯作者：
ROSNER, JL
大肠杆菌K-12的构造框架，单基因敲除突变体：Keio Collection。
影响因子：
9.9
作者：
通讯作者：
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