Expression of alternatively spliced isoforms of human Sp7 in osteoblast-like cells.
Expression of alternatively spliced isoforms of human Sp7 in osteoblast-like cells.
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DOI：
10.1186/1471-2164-4-43
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发表时间：
2003-11-07
期刊：
影响因子：
4.4
通讯作者：
Edgar AJ
中科院分区：
文献类型：
--
作者：
Milona MA;Gough JE;Edgar AJ
成骨细胞和软骨细胞分化涉及一系列协调的转录因子基因表达，以确定的时空方式调节增殖和基质蛋白的形成。骨形态发生蛋白-2诱导小鼠成骨细胞分化和骨形成所需的骨特异性蛋白-7 （Sp7）转录因子的表达。对其表达的调控可能有助于骨骼修复。Sp7是小鼠Osterix基因的人类同源物，定位于12q13.13，接近Sp1和同源盒基因簇- c。3-外显子基因的前两个外显子交替剪接，编码431个残基长蛋白异构体和一个氨基末端截断的413个残基短蛋白异构体。人类Sp7蛋白是Sp家族的一员，在Cys2-His2型和dna结合锌指中与Sp1有78%的同源性，但在其他地方几乎没有同源性。Sp7 mRNA在人胎儿成骨细胞、颅面成骨细胞、软骨细胞以及骨肉瘤细胞系HOS和MG63中表达，但在成人股骨成骨细胞中未检测到。一般来说，Sp7的短（或β）蛋白异构体的表达量远高于长（或α）蛋白异构体。在其他细胞类型的面板中未发现这两种异构体的表达。而在组织中，Sp7在睾丸、心脏、脑、胎盘、肺、胰腺、卵巢和脾脏中均检测到低水平。Sp7在人类中的表达主要局限于成骨细胞和软骨细胞，这两种细胞都与间充质细胞系不同。在这两种蛋白质异构体中，短异构体数量最多。
Osteogenic and chondrocytic differentiation involves a cascade of coordinated transcription factor gene expression that regulates proliferation and matrix protein formation in a defined temporo-spatial manner. Bone morphogenetic protein-2 induces expression of the murine Osterix/Specificity protein-7 (Sp7) transcription factor that is required for osteoblast differentiation and bone formation. Regulation of its expression may prove useful for mediating skeletal repair. Sp7, the human homologue of the mouse Osterix gene, maps to 12q13.13, close to Sp1 and homeobox gene cluster-C. The first two exons of the 3-exon gene are alternatively spliced, encoding a 431-residue long protein isoform and an amino-terminus truncated 413-residue short protein isoform. The human Sp7 protein is a member of the Sp family having 78% identity with Sp1 in the three, Cys2-His2 type, DNA-binding zinc-fingers, but there is little homology elsewhere. The Sp7 mRNA was expressed in human foetal osteoblasts and craniofacial osteoblasts, chondrocytes and the osteosarcoma cell lines HOS and MG63, but was not detected in adult femoral osteoblasts. Generally, the expression of the short (or beta) protein isoform of Sp7 was much higher than the long (or alpha) protein isoform. No expression of either isoform was found in a panel of other cell types. However, in tissues, low levels of Sp7 were detected in testis, heart, brain, placenta, lung, pancreas, ovary and spleen. Sp7 expression in humans is largely confined to osteoblasts and chondrocytes, both of which differentiate from the mesenchymal lineage. Of the two protein isoforms, the short isoform is most abundant.
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影响因子：
4.8
作者：
Jheon, AH;Ganss, B;Sodek, J
通讯作者：
Sodek, J
DOI：
10.1002/jbm.10020
发表时间：
2002-03-05
期刊：
JOURNAL OF BIOMEDICAL MATERIALS RESEARCH
影响因子：
--
作者：
Gough, JE;Christian, P;Jones, IA
通讯作者：
Jones, IA
影响因子：
2.9
作者：
Edgar AJ
通讯作者：
Edgar AJ
Osf2/Cbfa1：成骨细胞分化的转录激活因子
影响因子：
64.5
作者：
Ducy, P;Zhang, R;Karsenty, G
通讯作者：
Karsenty, G
影响因子：
64.8
作者：
Ducy, P;Desbois, C;Karsenty, G
通讯作者：
Karsenty, G