Functional analysis of cis-linked regulatory sequences in the HLA DRA promoter by transcription in vitro.
Functional analysis of cis-linked regulatory sequences in the HLA DRA promoter by transcription in vitro.
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通过体外转录对 HLA DRA 启动子中的顺式连接调节序列进行功能分析。
DOI：
10.1111/j.1399-0039.1990.tb01810.x
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Lee,JS
中科院分区：
文献类型：
--
作者：
Hume,CR;Lee,JS
Two consensus sequences, called × and Y boxes, capable of binding nuclear proteins and regulating expression in B cells have been defined within the immediate upstream region of major histocompatibility complex (MHC) class II promoters. Unlike other class II promoters, the HLA‐DR a (DRA) promoter also contains one element identical to the “octamer” motif of immunoglobulin variable region promoters that is responsible for B cell‐specific transcription. This “octamer” in the context of DRA appears capable of binding both the ubiquitous (OTF‐I) and lymphoid‐specific (OTF‐2) “octamer” binding proteins, but at least one other distinct “octamer” complex was found. In order to characterize the function of cis‐acting elements, we have developed anin vitrosystem in which a DRA promoter construct is transcribed more efficiently in extracts from B cells than in extracts from class II‐negative HeLa cells. 5′ deletion constructs which lacked the Y box, but retained the “octamer” the Y box reduced transcription by 95%. Using supercoiled, but not linear templates, we observed differences in transcription efficiencies from templates lacking or disrupting the × consensus element that reflect effects of random replacement of × box sequences in transient expression assays. Demonstration of the complete dependence on the Y box in this system suggests that, despite its demonstrated importance in the DRA promoter, the DRA “octamer” does not utilize OTF‐2 in a manner analogous to immunoglobulin promoters in B cells.
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使用流式细胞术进行基于微球的新型免疫荧光测定。
DOI：
--
发表时间：
1988
期刊：
JIM - Journal of Immunological Methods
影响因子：
--
作者：
M. Wilson;J. Wotherspoon
通讯作者：
J. Wotherspoon
蛋白质印迹分析中分离的 HIV 抗 p24 反应性的解释
DOI：
--
发表时间：
1987
期刊：
The Lancet
影响因子：
--
作者：
P. Lelie;C. Poel;H. Reesink
通讯作者：
H. Reesink
DOI：
10.1016/s0140-6736(87)92985-0
发表时间：
1987-09
期刊：
The Lancet
影响因子：
--
作者：
A. Ranki;Minerva Krohn;J. Allain;G. Franchini;S. Valle;J. Antonen;M. Leuther;K. Krohn
通讯作者：
A. Ranki;Minerva Krohn;J. Allain;G. Franchini;S. Valle;J. Antonen;M. Leuther;K. Krohn
通过输血传播人类免疫缺陷病毒 (HIV)，筛查结果为 HIV 抗体阴性。
影响因子：
158.5
作者：
J. W. Ward;S. Holmberg;J. R. Allen;D. Cohn;S. Critchley;S. Kleinman;B. Lenes;O. Ravenholt;Jacqualyn R. Davis;M. Quinn;H. Jaffe
通讯作者：
H. Jaffe
来自获得性免疫缺陷综合征逆转录病毒聚合酶 (pol) 基因核酸内切酶区域的重组多肽可检测大多数感染个体的血清抗体
影响因子：
5.4
作者：
K. Steimer;K. Higgins;M. Powers;J. Stephans;A. Gyenes;Carlos George;P. Luciw;Philip J. Barr;R. Hallewell;Ray Sanchez
通讯作者：
Ray Sanchez