Spatiotemporal Distribution and Assemblages of Planktonic Fungi in the Coastal Waters of the Bohai Sea.
Spatiotemporal Distribution and Assemblages of Planktonic Fungi in the Coastal Waters of the Bohai Sea.
复制标题
渤海沿岸海域浮游真菌时空分布与组合
DOI：
10.3389/fmicb.2018.00584
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发表时间：
2018
影响因子：
5.2
通讯作者：
Wang G
中科院分区：
文献类型：
--
作者：
Wang Y;Sen B;He Y;Xie N;Wang G
真菌在陆地和淡水生态系统的养分循环和生态功能中起着至关重要的作用。然而，它们在沿海生态系统中对应的许多生态方面在很大程度上仍然难以捉摸。利用高通量测序、定量PCR和环境数据分析，研究了渤海沿岸3个样带浮游真菌丰度和多样性的时空变化及其与生物和非生物相互作用。共鉴定出4362个ITS OTUs，其中60%以上为未分类真菌。在已分类的OTUs中，子囊菌门、担子菌门和壶菌门占主导地位，隐菌门和粘菌门（Mortierellales）偶有低优势。真菌特异性18S rRNA基因qPCR估计的平均丰度在4.28 × 106和1.13 × 107拷贝/L之间变化，不同样本间的丰度差异显著（P < 0.05），表明不同河流输入的潜在影响。子囊菌门和担子菌门OTU丰度的时空变化与河流输入和浮游植物碎屑的营养物质具有显著的一致性（P < 0.05）。8个主要真菌目中，Hypocreales的丰度在不同月份间差异极显著（P < 0.01）， Capnodiales、Pleosporales、Eurotiales和Sporidiobolales的丰度在不同断面间差异极显著（P < 0.05）。此外，我们的研究结果可能表明，在沿海水域中，隐菌门（高寄生虫）、壶菌门（寄生虫和腐养生物）和浮游植物之间存在三方相互作用模型。真菌网络除表现出合作和竞争关系外，还具有多个枢纽和重点otu。这些结果支持了浮游真菌在海洋生境中发挥多种生态作用的观点，并进一步概述了生态位过程、三方和共现相互作用是其群落结构和时空分布的主要驱动力。
Fungi play a critical role in the nutrient cycling and ecological function in terrestrial and freshwater ecosystems. Yet, many ecological aspects of their counterparts in coastal ecosystems remain largely elusive. Using high-throughput sequencing, quantitative PCR, and environmental data analyses, we studied the spatiotemporal changes in the abundance and diversity of planktonic fungi and their abiotic and biotic interactions in the coastal waters of three transects along the Bohai Sea. A total of 4362 ITS OTUs were identified and more than 60% of which were unclassified Fungi. Of the classified OTUs three major fungal phyla, Ascomycota, Basidiomycota, and Chytridiomycota were predominant with episodic low dominance phyla Cryptomycota and Mucoromycota (Mortierellales). The estimated average Fungi-specific 18S rRNA gene qPCR abundances varied within 4.28 × 106 and 1.13 × 107copies/L with significantly (P < 0.05) different abundances among the transects suggesting potential influence of the different riverine inputs. The spatiotemporal changes in the OTU abundance of Ascomycota and Basidiomycota phyla coincided significantly (P < 0.05) with nutrients traced to riverine inputs and phytoplankton detritus. Among the eight major fungal orders, the abundance of Hypocreales varied significantly (P < 0.01) across months while Capnodiales, Pleosporales, Eurotiales, and Sporidiobolales varied significantly (P < 0.05) across transects. In addition, our results likely suggest a tripartite interaction model for the association within members of Cryptomycota (hyperparasites), Chytridiomycota (both parasites and saprotrophs), and phytoplankton in the coastal waters. The fungal network featured several hubs and keystone OTUs besides the display of cooperative and competitive relationship within OTUs. These results support the notion that planktonic fungi, hitherto mostly undescribed, play diverse ecological roles in marine habitats and further outline niche processes, tripartite and co-occurrence interaction as the major drivers of their community structure and spatiotemporal distribution in the coastal water column.
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影响因子：
2.8
作者：
Danovaro, Roberto;Pusceddu, Antonio
通讯作者：
Pusceddu, Antonio
影响因子：
5.1
作者：
Hassett, B. T.;Gradinger, R.
通讯作者：
Gradinger, R.
影响因子：
4.3
作者：
Faust K;Sathirapongsasuti JF;Izard J;Segata N;Gevers D;Raes J;Huttenhower C
通讯作者：
Huttenhower C
影响因子：
5.2
作者：
Gleason FH;Lilje O;Marano AV;Sime-Ngando T;Sullivan BK;Kirchmair M;Neuhauser S
通讯作者：
Neuhauser S
DOI：
10.1007/978-3-319-29137-6_5
发表时间：
2016-01-01
期刊：
BIOLOGY OF MICROFUNGI
影响因子：
--
作者：
Benny, Gerald L.;Smith, Matthew E.;White, Merlin M.
通讯作者：
White, Merlin M.