Molecular cloning of porcine Siglec-3, Siglec-5 and Siglec-10, and identification of Siglec-10 as an alternative receptor for porcine reproductive and respiratory syndrome virus (PRRSV).
Molecular cloning of porcine Siglec-3, Siglec-5 and Siglec-10, and identification of Siglec-10 as an alternative receptor for porcine reproductive and respiratory syndrome virus (PRRSV).
复制标题
DOI：
10.1099/jgv.0.000859
复制
发表时间：
2017-08
期刊：
影响因子：
--
通讯作者：
Nauwynck HJ
中科院分区：
文献类型：
--
作者：
Xie J;Christiaens I;Yang B;Breedam WV;Cui T;Nauwynck HJ
近年来，猪繁殖与呼吸综合征病毒（PRRSV）的几种进入介质已被表征。猪唾液酸粘附素[pSn，也称为唾液酸结合免疫球蛋白型凝集素（Siglec-1）]和猪CD 163（pCD 163）已被鉴定为最重要的宿主进入介质，其可以完全协调PRRSV感染进入巨噬细胞。然而，最近的分离株不仅表现出对唾液酸粘附素阳性细胞的嗜性，而且还表现出对唾液酸粘附素阴性细胞的嗜性。这一观察结果可能部分解释了额外的受体，可以支持PRRSV的结合和进入的存在。在寻找新受体的过程中，克隆并表征了最近鉴定的猪Siglec（Siglec-3、Siglec-5和Siglec-10），它们是与唾液酸粘附素相同家族的成员。只有Siglec-10能够显著改善CD 163转染细胞系中的PRRSV感染和产生。与唾液酸粘附素相比，Siglec-10作为PRRSV 2型毒株MN-184的受体同样有效，但其支持PRRSV 1型毒株LV（莱利斯塔病毒）感染的能力较低。Siglec-10被证明参与了PRRSV的内吞作用，证实了Siglec-10在PRRSV进入过程中的重要作用。综上所述，可以说，PRRSV可能利用几种Siglecs进入巨噬细胞，这可能解释了致病机制的菌株差异。
In recent years, several entry mediators have been characterized for porcine reproductive and respiratory syndrome virus (PRRSV). Porcine sialoadhesin [pSn, also known as sialic acid-binding immunoglobulin-type lectin (Siglec-1)] and porcine CD163 (pCD163) have been identified as the most important host entry mediators that can fully coordinate PRRSV infection into macrophages. However, recent isolates have not only shown a tropism for sialoadhesin-positive cells, but also for sialoadhesin-negative cells. This observation might be partly explained by the existence of additional receptors that can support PRRSV binding and entry. In the search for new receptors, recently identified porcine Siglecs (Siglec-3, Siglec-5 and Siglec-10), members of the same family as sialoadhesin, were cloned and characterized. Only Siglec-10 was able to significantly improve PRRSV infection and production in a CD163-transfected cell line. Compared with sialoadhesin, Siglec-10 performed equally effectively as a receptor for PRRSV type 2 strain MN-184, but it was less capable of supporting infection with PRRSV type 1 strain LV (Lelystad virus). Siglec-10 was demonstrated to be involved in the endocytosis of PRRSV, confirming the important role of Siglec-10 in the entry process of PRRSV. In conclusion, it can be stated that PRRSV may use several Siglecs to enter macrophages, which may explain the strain differences in the pathogenesis.
登录
查看更多内容
Nidoviral家族Arteriviridae的重组和扩展。
影响因子：
2.7
作者：
Kuhn JH;Lauck M;Bailey AL;Shchetinin AM;Vishnevskaya TV;Bào Y;Ng TF;LeBreton M;Schneider BS;Gillis A;Tamoufe U;Diffo Jle D;Takuo JM;Kondov NO;Coffey LL;Wolfe ND;Delwart E;Clawson AN;Postnikova E;Bollinger L;Lackemeyer MG;Radoshitzky SR;Palacios G;Wada J;Shevtsova ZV;Jahrling PB;Lapin BA;Deriabin PG;Dunowska M;Alkhovsky SV;Rogers J;Friedrich TC;O'Connor DH;Goldberg TL
通讯作者：
Goldberg TL
影响因子：
7.2
作者：
Oeckinghaus, Andrea;Ghosh, Sankar
通讯作者：
Ghosh, Sankar
影响因子：
3.9
作者：
Crocker, Paul R.;Redelinghuys, Pierre
通讯作者：
Redelinghuys, Pierre
DOI：
10.1126/science.1168988
发表时间：
2009-03-27
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Chen GY;Tang J;Zheng P;Liu Y
通讯作者：
Liu Y
影响因子：
2.9
作者：
Alvarez, B.;Escalona, Z.;Dominguez, J.
通讯作者：
Dominguez, J.