Defining Composition and Function of the Rhizosphere Microbiota of Barley Genotypes Exposed to Growth-Limiting Nitrogen Supplies.
Defining Composition and Function of the Rhizosphere Microbiota of Barley Genotypes Exposed to Growth-Limiting Nitrogen Supplies.
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DOI：
10.1128/msystems.00934-22
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发表时间：
2022-12-20
期刊：
影响因子：
6.4
通讯作者：
中科院分区：
文献类型：
--
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根际是根与土壤的交界面，在根际的微生物群可以调节植物的生长、发育和健康。这些微生物群落不是随机地从周围的土壤中聚集起来的，但它们的组成和假定的功能至少部分地由寄主植物控制。在这里，我们使用主食谷物大麦作为模型，以获得对氮的不同施用的影响的新见解，氮是全球作物生产的限速步骤，对根际微生物群的宿主遗传控制。利用高通量扩增子测序调查，我们确定植物吸收氮的有效性是促进野生和驯化大麦基因型根际个别分类群选择性富集的一个因素。霰弹枪测序和宏基因组组装基因组表明，这种分类多样化反映在功能特化上，表现为暴露于氮条件下限制大麦生长的植物微生物群的多个基因本体术语的差异富集。最后，一项植物土壤反馈实验显示，大麦微生物群的宿主控制支撑了系统发育多样性细菌群的组装，这些细菌群被认为是在氮限制供应下维持植物性能所必需的。综上所述，我们的观察结果表明，在限制植物生长的氮条件下，宿主-微生物和微生物-微生物的相互作用在分类和功能水平上微调了大麦微生物群的宿主遗传选择。这些招募线索的破坏会对植物生长产生负面影响。根际是植物根系周围的一层薄薄的土壤，生活在根际的微生物群可以促进寄主植物的生长、发育和健康。先前的研究表明，寄主植物遗传组成的差异与根际微生物群组成的变化一致。当观察与需要投入的现代栽培品种及其在边缘条件下进化的野生近缘种相关的微生物群时，这一点尤为明显。然而，这些差异的功能意义仍有待充分阐明。我们研究了全球作物大麦的野生和栽培基因型的根际微生物群，并确定限制植物生长的营养条件增强了宿主对根-土界面微生物的控制。这反映在植物和基因型依赖的根际微生物群的功能专门化上，这似乎是最佳植物生长所必需的。这些发现为根际微生物群对植物生长和可持续农业的重要性提供了新的见解。
The microbiota populating the rhizosphere, the interface between roots and soil, can modulate plant growth, development, and health. These microbial communities are not stochastically assembled from the surrounding soil, but their composition and putative function are controlled, at least partially, by the host plant. Here, we use the staple cereal barley as a model to gain novel insights into the impact of differential applications of nitrogen, a rate-limiting step for global crop production, on the host genetic control of the rhizosphere microbiota. Using a high-throughput amplicon sequencing survey, we determined that nitrogen availability for plant uptake is a factor promoting the selective enrichment of individual taxa in the rhizosphere of wild and domesticated barley genotypes. Shotgun sequencing and metagenome-assembled genomes revealed that this taxonomic diversification is mirrored by a functional specialization, manifested by the differential enrichment of multiple Gene Ontology terms, of the microbiota of plants exposed to nitrogen conditions limiting barley growth. Finally, a plant soil feedback experiment revealed that host control of the barley microbiota underpins the assembly of a phylogenetically diverse group of bacteria putatively required to sustain plant performance under nitrogen-limiting supplies. Taken together, our observations indicate that under nitrogen conditions limiting plant growth, host-microbe and microbe-microbe interactions fine-tune the host genetic selection of the barley microbiota at both taxonomic and functional levels. The disruption of these recruitment cues negatively impacts plant growth. IMPORTANCE The microbiota inhabiting the rhizosphere, the thin layer of soil surrounding plant roots, can promote the growth, development, and health of their host plants. Previous research indicated that differences in the genetic composition of the host plant coincide with variations in the composition of the rhizosphere microbiota. This is particularly evident when looking at the microbiota associated with input-demanding modern cultivated varieties and their wild relatives, which have evolved under marginal conditions. However, the functional significance of these differences remains to be fully elucidated. We investigated the rhizosphere microbiota of wild and cultivated genotypes of the global crop barley and determined that nutrient conditions limiting plant growth amplify the host control on microbes at the root-soil interface. This is reflected in a plant- and genotype-dependent functional specialization of the rhizosphere microbiota, which appears to be required for optimal plant growth. These findings provide novel insights into the significance of the rhizosphere microbiota for plant growth and sustainable agriculture.
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DOI：
10.1038/ismej.2011.159
发表时间：
2012-05
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
DOI：
10.1038/ismej.2012.8
发表时间：
2012-08
期刊：
The ISME journal
影响因子：
--
作者：
通讯作者：
--
影响因子：
48
作者：
Callahan BJ;McMurdie PJ;Rosen MJ;Han AW;Johnson AJ;Holmes SP
通讯作者：
Holmes SP
影响因子：
0.8
作者：
Robertson-Albertyn S;Abbott JC;Concas F;Brown LH;Orr JN;George TS;Bulgarelli D
通讯作者：
Bulgarelli D
影响因子：
9.4
作者：
ABBOTT, LK;ROBSON, AD;DEBOER, G
通讯作者：
DEBOER, G