Optimization of cationic (Q)-paper for detection of arboviruses in infected mosquitoes.
Optimization of cationic (Q)-paper for detection of arboviruses in infected mosquitoes.
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DOI：
10.1016/j.jviromet.2018.08.004
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发表时间：
2018-11
影响因子：
3.1
通讯作者：
Benner SA
中科院分区：
文献类型：
--
作者：
Glushakova LG;Alto BW;Kim MS;Wiggins K;Eastmond B;Moussatche P;Burkett-Cadena ND;Benner SA
Previously, a cellulose-based cationic (Q) paper derivatized with quaternary ammonium groups was shown to be a convenient platform to collect, preserve, and store nucleic acids (NAs) derived from mosquito vectors infected with pathogens for surveillance. NAs bind electrostatically to Q-paper, but the quantity of NA bound depends on the paper’s binding capacity. To optimize the original technology for mosquito surveillance, factors that affected NA absorbance on Q-paper were evaluated. Sixteen variations of Q-paper were prepared with modifications of the derivatizing reagents and derivatization temperature. The binding capacities of these variations were determined first with 1,3,5-benzenetricarboxylic (BTCA), then viral RNA (purified or in infected mosquito samples) was used for validation. For this, samples with Zika (ZIKV) and chikungunya (CHIKV) RNA or virus-infected Aedes aegypti mosquito bodies were applied to sixteen Q-paper variants. Washing the paper samples with water versus elution with aqueous salt (1 M) gave samples that were analyzed for viral RNA by a PCR-based direct Luminex hybridization assay. The comparison ranked the Q-paper binding capacities from the lowest to the highest. The Q-paper with the highest RNA binding capability was further validated with ZIKV- and CHIKV-infected mosquito saliva.
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影响因子：
7.4
作者：
Yang, Zunyi;Durante, Michael;Glushakova, Lyudmyla G.;Sharma, Nidhi;Leal, Nicole A.;Bradley, Kevin M.;Chen, Fei;Benner, Steven A.
通讯作者：
Benner, Steven A.
影响因子：
3.1
作者：
Glushakova LG;Alto BW;Kim MS;Bradley A;Yaren O;Benner SA
通讯作者：
Benner SA
影响因子：
2.8
作者：
Powell JR;Tabachnick WJ
通讯作者：
Tabachnick WJ
影响因子：
2.1
作者：
Benedict, Mark Q.;Levine, Rebecca S.;Lounibos, L. Philip
通讯作者：
Lounibos, L. Philip
DOI：
10.4269/ajtmh.1984.33.176
发表时间：
1984-01-01
影响因子：
3.3
作者：
TURELL, MJ;GARGAN, TP;BAILEY, CL
通讯作者：
BAILEY, CL